WB (Western Blot)
(Western blot analysis of lysates from Rabbit IgG using HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb (AAA283340) at 1:1000-1:20000 dilution incubated overnight at 4?.Lysates/proteins: 100ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
IgG Secondary Antibody | IGHG secondary antibody
HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
Reactivity
Rabbit
Applications
ELISA, Western Blot
Purity
Affinity purification
Synonyms
IgG; N/A; HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb; IGHG secondary antibody
Host
Mouse
Reactivity
Rabbit
Isotype
Mouse IgG1
Purity/Purification
Affinity purification
Form/Format
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Applicable Applications for IGHG secondary antibody
ELISA, WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 96-323 of rabbit IgG (Fc)(P01870).
Conjugation
HRP
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from Rabbit IgG using HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb (AAA283340) at 1:1000-1:20000 dilution incubated overnight at 4?.Lysates/proteins: 100ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of lysates from Rabbit IgG using HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb (AAA283340) at 1:1000-1:20000 dilution incubated overnight at 4?.Lysates/proteins: 100ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Application Data
(Dose response curve of HRP conjugated Mouse Anti-Rabbit IgG (Fc) mAb measured by ELISA. 1 ug/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 uL of HRP conjugated Mouse Anti-Rabbit IgG (Fc) mAb in a 2 fold serial dilution from 2 ug/mL to 1.22*10^-4 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated thatMouse Anti-Rabbit IgG (Fc) mAb has highly specific recognition of Rabbit IgG while no or minimal cross reactivity to Rabbit IgM? Human IgG?Mouse IgG?Rat IgG.)
Application Data
(Dose response curve of HRP conjugated Mouse Anti-Rabbit IgG (Fc) mAb measured by ELISA. 1 ug/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 uL of HRP conjugated Mouse Anti-Rabbit IgG (Fc) mAb in a 2 fold serial dilution from 2 ug/mL to 1.22*10^-4 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated thatMouse Anti-Rabbit IgG (Fc) mAb has highly specific recognition of Rabbit IgG while no or minimal cross reactivity to Rabbit IgM? Human IgG?Mouse IgG?Rat IgG.)
Related Product Information for IGHG secondary antibody
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies. Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Product Categories/Family for IGHG secondary antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The IGHG (Catalog #AAA283340) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the IGHG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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