Mouse Involucrin Antibody | anti-IVL antibody

Involucrin (Squamous Cell Terminal Differentiation Marker)

Cat. Num. AAA215047

• Reactivity: Human, Gorilla, Owl Monkey, Pig, Dog; Does not react with Mouse.
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Involucrin; N/A; Involucrin (Squamous Cell Terminal Differentiation Marker); IVL; anti-IVL antibody

• Host: Mouse
• Reactivity: Human, Gorilla, Owl Monkey, Pig, Dog; Does not react with Mouse.
• Isotype: IgG1, kappa
• Clone Number: rIVRN/827
• Specificity: It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 585

• Applicable Applications for anti-IVL antibody: IHC (Immunohistochemistry)
• Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
• Immunogen: Recombinant human full-length Involucrin protein (IVRN)
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 1q21.3
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified Involucrin Mouse Recombinant Monoclonal Antibody (rIVRN/827). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Skin stained with Involucrin Mouse Recombinant Monoclonal Antibody (rIVRN/827).)

References

Hudson DL, et. al. Hybridoma, 1992, 11:367-79

NCBI and Uniprot Product Information

• NCBI GI #: 44890059
• NCBI GeneID: 3713
• NCBI Accession #: NP_005538.2
• NCBI GenBank Nucleotide #: NP_005538.2
• UniProt Accession #: P07476
• Molecular Weight: 66-170kDa
• NCBI Official Full Name: involucrin
• NCBI Official Synonym Full Names: involucrin
• NCBI Official Symbol: IVL
• NCBI Protein Information: involucrin
• UniProt Protein Name: Involucrin
• UniProt Gene Name: IVL
• UniProt Entry Name: INVO_HUMAN