Chlamydia trachomatis LGV Type-2 (Strain 434) Elementary Bodies Native Protein
C. trachomatis LGV Type-2 EB
Purity
Mouse L cells are infected with C. trachomatis elementary bodies. Optimally infected cells are harvested and disrupted by sonication in PBS. Cellular debris is removed using centrifugation.
Synonyms
Chlamydia trachomatis LGV Type-2 (Strain 434) Elementary Bodies; N/A; C. trachomatis LGV Type-2 EB; Chlamydia trachomatis LGV Type 2, Elementary bodies (EB); Chlamydia trachomatis LGV Type-2 (Strain 434) Elementary Bodies native protein
Host
Mouse L cells
Chlamydia trachomatis LGV type II
Strain: 434
Chlamydia trachomatis LGV type II
Strain: 434
Specificity
Chlamydia trachomatis LGV Type-2 (Strain 434) Elementary Bodies
Purity/Purification
Mouse L cells are infected with C. trachomatis elementary bodies. Optimally infected cells are harvested and disrupted by sonication in PBS. Cellular debris is removed using centrifugation.
Form/Format
Purified, Liquid
Concentration
4.9 mg/ml (dye binding microassay) (minimum concentration 1mg/ml)
Titer is controlled lot-to-lot by ELISA IgG reactivity. (varies by lot)
Titer is controlled lot-to-lot by ELISA IgG reactivity. (varies by lot)
Inactivation
Inactivated using famma radiation. Effective primarily by damaging chlamydial genetic material. Effectiveness of inactivation is tested by inoculating an L cell monolayer with antigen. The culture is manipulated using the original optimal culture conditions used to manufacture the antigen. The culture is monitored for cytopathic effect for 5 days. If no signs of infection are observed the culture is passaged into a fresh monolayer. The second passage is monitored for a further 5 day period. If no cytopathic effect is observed in either passage the antigen is considered inactivated. As result: No growth deteced.
Buffer
PBS, pH 7.2
Preservative
No
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Warnings
No test guarantees a product to be non-infectious. All materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for biological materials should be employed when handling this product.
Preparation and Storage
Store at < -70 degree C. Avoid multiple freeze/thaw cycles.
Related Product Information for Chlamydia trachomatis LGV Type-2 (Strain 434) Elementary Bodies native protein
Should be sonicated immediately prior to use to ensure that the preparation is uniform. Used for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases and M capture formats. Each laboratory should determined an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Product Categories/Family for Chlamydia trachomatis LGV Type-2 (Strain 434) Elementary Bodies native protein
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Product Notes
The Chlamydia trachomatis LGV Type-2 (Strain 434) Elementary Bodies (Catalog #AAA58245) is a Native Protein produced from Mouse L cells Chlamydia trachomatis LGV type II Strain: 434 and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Chlamydia trachomatis LGV Type-2 (Strain 434) Elementary Bodies, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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