Mouse Tenascin C Antibody | anti-TNC antibody

Tenascin C (Stromal Marker for Epithelial Malignancy)

Cat. Num. AAA215017

• Gene Names: TNC; GP; JI; TN; HXB; GMEM; TN-C; DFNA56; 150-225
• Reactivity: Human; Does not react with Rat.
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Tenascin C; N/A; Tenascin C (Stromal Marker for Epithelial Malignancy); Cytotactin; Glioma-associated-extracellular matrix antigen; GMEM; GP 150-225; Hexabrachion (HXB); JI; Myotendinous antigen; Neuronectin; TNC; anti-TNC antibody

• Host: Mouse
• Reactivity: Human; Does not react with Rat.
• Isotype: IgG1, kappa
• Clone Number: TNC/3635
• Specificity: In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 2201

• Applicable Applications for anti-TNC antibody: IHC (Immunohistochemistry)
• Positive Control: Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell and lung carcinomas. Staining of normal fibroblasts serves as internal positive control (IHC).
• Immunogen: Recombinant human full-length TNC protein
• Cellular Localization: Connective tissue matrix
• Hu-chromosome Location: 9q33
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified Tenascin C Mouse Recombinant Monoclonal Antibody (TNC/3635). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Tenascin C Mouse Recombinant Monoclonal Antibody (TNC/3635).)

References

Verstraeten AA, et. al. British Journal of Dermatology, 1992, 127(6):571-4.

NCBI and Uniprot Product Information

• NCBI GI #: 153946395
• NCBI GeneID: 3371
• NCBI Accession #: NP_002151.2
• NCBI GenBank Nucleotide #: NP_002151.2
• UniProt Accession #: P24821
• Molecular Weight: 210kDa and 300kDa
• NCBI Official Full Name: tenascin
• NCBI Official Synonym Full Names: tenascin C
• NCBI Official Symbol: TNC
• NCBI Official Synonym Symbols: GP; JI; TN; HXB; GMEM; TN-C; DFNA56; 150-225
• NCBI Protein Information: tenascin
• UniProt Protein Name: Tenascin
• UniProt Gene Name: TNC
• UniProt Synonym Gene Names: HXB; TN; TN-C
• UniProt Entry Name: TENA_HUMAN