Application Data
(Figure 2. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin mouse mAb with DAB staining.)
Mouse VIMENTIN Antibody | anti-VIM antibody
VIMENTIN Antibody
Reactivity
Human, Monkey
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
VIMENTIN; N/A; VIMENTIN Antibody; anti-VIM antibody
Host
Mouse
Reactivity
Human, Monkey
Isotype
IgG1
Clone Number
4F2E9
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
466
Applicable Applications for anti-VIM antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Application Data
(Figure 2. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin mouse mAb with DAB staining.)
Application Data
(Figure 2. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin mouse mAb with DAB staining.)
Application Data
(Figure 1. Western blot analysis using Vimentin mouse mAb against Hela (1), COS (2), HEK293 (3) and U20S (4) cell lysate.)
Application Data
(Figure 1. Western blot analysis using Vimentin mouse mAb against Hela (1), COS (2), HEK293 (3) and U20S (4) cell lysate.)
Related Product Information for anti-VIM antibody
Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential identification of undifferentiated neoplasms and malignant tumors.
Product Categories/Family for anti-VIM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54kDa
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Protein Information
vimentin
UniProt Protein Name
Vimentin
UniProt Gene Name
VIM
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Product Notes
The VIM vim (Catalog #AAA108585) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The VIMENTIN Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's VIMENTIN can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the VIM vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VIMENTIN, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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