Standard Curve (Sample)
Bovine Myeloperoxidase ELISA Kit | MPO elisa kit
Bovine Myeloperoxidase ELISA Kit
Reactivity
Bovine
Synonyms
Myeloperoxidase; N/A; Bovine Myeloperoxidase ELISA Kit; MPO elisa kit
Reactivity
Bovine
Samples
Mouse Plasma.
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-assay Precision
These studies are currently in progress. Please contact us for more information.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MPO elisa kit
Intended Uses: This mouse prekallikrein total antigen assay is intended for the quantitative determination of total prekallikrein antigen in mouse plasma. For research use only.
Principle of the Assay: Mouse prekallikrein will bind to the monoclonal capture antibody coated on the microtiter plate. Prekallikrein and kallikrein will react with the antibody on the plate. After appropriate washing steps, anti-mouse prekallikrein primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse prekallikrein. Color development is proportional to the concentration of total prekallikrein in the samples!!Background/Introduction: Prekallikrein is the glycosylated single chain zymogen precursor of the plasma serine protease kallikrein. Plasma prekallikrein circulates with kininogen and is activated by Factor XIIa in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time [1]. Elevated plasma prekallikrein is associated with diabetes [2] and cardiovascular disease [3].
Principle of the Assay: Mouse prekallikrein will bind to the monoclonal capture antibody coated on the microtiter plate. Prekallikrein and kallikrein will react with the antibody on the plate. After appropriate washing steps, anti-mouse prekallikrein primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse prekallikrein. Color development is proportional to the concentration of total prekallikrein in the samples!!Background/Introduction: Prekallikrein is the glycosylated single chain zymogen precursor of the plasma serine protease kallikrein. Plasma prekallikrein circulates with kininogen and is activated by Factor XIIa in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time [1]. Elevated plasma prekallikrein is associated with diabetes [2] and cardiovascular disease [3].
Product Categories/Family for MPO elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
87,248 Da
NCBI Official Full Name
myeloperoxidase
NCBI Official Synonym Full Names
myeloperoxidase
NCBI Official Symbol
MPO
NCBI Protein Information
myeloperoxidase
UniProt Protein Name
Myeloperoxidase
UniProt Gene Name
MPO
UniProt Synonym Gene Names
MPO
UniProt Entry Name
PERM_HUMAN
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Product Notes
The Bovine MPO mpo (Catalog #AAA91824) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA91824 ELISA Kit recognizes Bovine MPO. It is sometimes possible for the material contained within the vial of "Myeloperoxidase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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