Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse MPO. No significant cross-reactivity or interference between mouse MPO and analogues was observed.
Sequence Length
718
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
< 7.8 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for MPO elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPO bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
81,182 Da
NCBI Official Full Name
myeloperoxidase
NCBI Official Synonym Full Names
myeloperoxidase
NCBI Official Symbol
Mpo
NCBI Official Synonym Symbols
mKIAA4033
NCBI Protein Information
myeloperoxidase
UniProt Protein Name
Myeloperoxidase
UniProt Gene Name
Mpo
UniProt Synonym Gene Names
MPO
UniProt Entry Name
PERM_MOUSE