SDS-PAGE
(Fig. SDS-PAGE analysis of Human CD20 protein.)
CD20 recombinant protein
Human CD20 protein, His tag (Animal-Free)
Gene Names
MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Purity
>95% as determined by SDS-PAGE
Synonyms
CD20; N/A; Human CD20 protein, His tag (Animal-Free); CD80; Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen; T-lymphocyte activation antigen CD80; CD20 recombinant protein
Host
HEK293 cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
PBS, pH7.4.
Sequence Positions
91aa
Sequence
Amino acid sequence derived from human CD20 (NP_068769.2) (Glu213-Pro297) was expressed with a 6 His tag at the C-terminus
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method.
Protein Construction
Amino acid sequence derived from human CD20 (NP_068769.2) (Glu213-Pro297) was expressed with a 6 His tag at the C-terminus.
Usage Notes
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD20 protein alpha protein using the buffer we provided not less than 100 ug/mL, which can then be further diluted to other aqueous solutions.
Protein Length
The recombinant human consists of 91 amino acids and predicts a molecular mass of 10.6 kDa. It migrates as an aproximately 23 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
Preparation and Storage
Lyophilized Human CD20 protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
SDS-PAGE
(Fig. SDS-PAGE analysis of Human CD20 protein.)
SDS-PAGE
(Fig. SDS-PAGE analysis of Human CD20 protein.)
Related Product Information for CD20 recombinant protein
Background: B-lymphocyte antigen CD20 is also known as B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1 and MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. CD20/MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20/MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.
Product Categories/Family for CD20 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10.6 KD
NCBI Official Full Name
B-lymphocyte antigen CD20
NCBI Official Synonym Full Names
membrane spanning 4-domains A1
NCBI Official Symbol
MS4A1
NCBI Official Synonym Symbols
B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
NCBI Protein Information
B-lymphocyte antigen CD20
UniProt Protein Name
B-lymphocyte antigen CD20
UniProt Gene Name
MS4A1
UniProt Synonym Gene Names
CD20
UniProt Entry Name
CD20_HUMAN
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Product Notes
The CD20 ms4a1 (Catalog #AAA332475) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 91aa. The amino acid sequence is listed below: Amino acid sequence derived from human CD20 (NP_068769.2) (Glu213-Pr o297) was expressed with a 6 His tag at the C-terminus. It is sometimes possible for the material contained within the vial of "CD20, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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