Standard Curve (Sample)
Human Mesothelin ELISA Kit | MSLN elisa kit
Human Mesothelin ELISA Kit
Gene Names
MSLN; MPF; SMRP
Reactivity
Human
Applications
ELISA
Synonyms
Mesothelin; N/A; Human Mesothelin ELISA Kit; MPF; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; MSLN elisa kit
Reactivity
Human
Specificity
The Human Mesothelin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Mesothelin proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
630
Samples
Cell Culture Supernatants, Serum, Plasma, Urine.
Sensitivity
10 pg/ml.
Applicable Applications for MSLN elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MSLN elisa kit
Principle of the Assay||The Human Mesothelin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Mesothelin in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Human Mesothelin coated on a 96-well plate. Standards and samples are pipetted into the wells and Mesothelin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Mesothelin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mesothelin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.
Background/Introduction: Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
67,940 Da
NCBI Official Full Name
Mesothelin
NCBI Official Synonym Full Names
mesothelin
NCBI Official Symbol
MSLN
NCBI Official Synonym Symbols
MPF; SMRP
NCBI Protein Information
mesothelin
UniProt Protein Name
Mesothelin
UniProt Gene Name
MSLN
UniProt Synonym Gene Names
MPF; MPF
UniProt Entry Name
MSLN_HUMAN
Similar Products
Product Notes
The Human MSLN msln (Catalog #AAA261673) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261673 ELISA Kit recognizes Human MSLN. AAA Biotech's Mesothelin can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the MSLN msln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mesothelin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.