MTSS1 recombinant protein

Recombinant Human MTSS1 Protein (aa1-250, His & MBP tag)

Cat. Num. AAA173533

• Gene Names: MTSS1; MIM; MIMA; MIMB
• Purity: > 80 % as determined by reducing SDS-PAGE.
• Synonyms: MTSS1; N/A; Recombinant Human MTSS1 Protein (aa1-250, His & MBP tag); DKFZp781P2223, FLJ44694, KIAA0429, MIM, MIMA, MIMB; MTSS1 recombinant protein

• Host: E.coli
• Purity/Purification: > 80 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.5, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence Positions: Met 1-Ser 250

• Species: Human
• Tag: N-His & MBP
• Reconstitution: It is recommended that sterile water (80uL) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; ; Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for MTSS1 recombinant protein

Background: MTSS1 (Metastasis suppressor 1), also known as Missing in metastasis (MIM), is a tissue-specific regulator of plasma membrane dynamics. MTSS1 is well described for its function as a metastasis suppressor gene and is expressed in a variety of tissues. MTSS1 might be involved in shaping neuronal membranes in vivo. MTSS1 deforms phosphoinositide-rich membranes through its I-BAR domain and interacts with actin monomers through its WH2 domain. MTSS1/MIM was first identified as a metastasis suppressor missing in metastatic bladder carcinoma cell lines. MTSS1 is a prognostic indicator of disease-free survival in breast cancer patients and demonstrates the ability to play a role in governing the metastatic nature of breast cancer cells. MTSS1 may serve as a useful biomarker for the prediction of outcome of gastric cancer. The down-regulation of MTSS1 that may be caused by DNA methylation was also observed in many other type s of cancer.Recent work proposed that MIM also potentiates Sonic hedgehog (Shh)-induced gene expression. MTSS1 as a multiple functional molecular player and has an important role in development, carcinogenesis and metastasis.

Description: A DNA sequence encoding the human MTSS1 (EAW92073.1) N-terminal fragment (Met 1-Ser 250) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 119612479
• NCBI GeneID: 9788
• Molecular Weight: Mol_Mass: 71.8 kDa; AP_Mol_Mass: 66 kDa
• NCBI Official Full Name: metastasis suppressor 1, isoform CRA_e
• NCBI Official Synonym Full Names: MTSS1, I-BAR domain containing
• NCBI Official Symbol: MTSS1
• NCBI Official Synonym Symbols: MIM; MIMA; MIMB
• NCBI Protein Information: metastasis suppressor protein 1
• UniProt Protein Name: Metastasis suppressor protein 1
• UniProt Gene Name: MTSS1
• UniProt Synonym Gene Names: KIAA0429; MIM