Murine LIF ELISA Kit | LIF elisa kit

Murine LIF Pre-Coated ELISA Kit

Cat. Num. AAA79374

• Reactivity: Murine
• Synonyms: LIF; N/A; Murine LIF Pre-Coated ELISA Kit; LIF elisa kit

• Reactivity: Murine
• Specificity: Natural and recombinant Murine LIF Pre-Coated ELISA Kit
• Assay Type: Solid Phase Sandwich ELISA: 96-Well Strip Plate
• Detection Range: 7.8pg/ml-500pg/ml
• Sensitivity: <10pg/ml
• Components: Anti-Murine LIF Pre-coated 96-well strip microplate; Murine LIF Standard; Murine LIF Biotinylated antibody (100x); Avidin-Biotin-Peroxidase Complex (100x); Sample Diluent; Antibody Diluent; Avidin-Biotin-Peroxidase Diluent; Wash Buffer Concentrate (Powder for 1000mL); Color Developing Reagent; Stop Solution; Plate Sealers
• Interference: No detectable cross-reactivity with other relevant proteins

• Preparation and Storage: Store the unopened product at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for LIF elisa kit

LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor.
Murine LIF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure LIF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for LIF. It contains E Coli-expressed recombinant LIF. The detection antibody is a biotinylated goat polyclonal antibody specific for LIF. The kit is analytically validated with ready to use reagents.

NCBI and Uniprot Product Information

• NCBI GI #: 88014736
• NCBI GeneID: 16878
• NCBI Accession #: NP_001034626.1
• NCBI GenBank Nucleotide #: NM_001039537.2
• UniProt Accession #: P09056
• Molecular Weight: 22,287 Da
• NCBI Official Full Name: leukemia inhibitory factor isoform b
• NCBI Official Synonym Full Names: leukemia inhibitory factor
• NCBI Official Symbol: Lif
• NCBI Protein Information: leukemia inhibitory factor; d factor; differentiation-stimulating factor
• UniProt Protein Name: Leukemia inhibitory factor
• UniProt Gene Name: Lif
• UniProt Synonym Gene Names: LIF; D factor
• UniProt Entry Name: LIF_MOUSE