Standard Curve (Sample)
Human myosin light chain kinase ELISA Kit | MYLK elisa kit
Human myosin light chain kinase, MLCK ELISA Kit
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Reactivity
Human
Synonyms
myosin light chain kinase; N/A; Human myosin light chain kinase, MLCK ELISA Kit; Human myosin light chain kinase; MLCK ELISA Kit; DKFZp686I10125; FLJ12216; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK1; smMLCK; OTTHUMP00000180642; myosin; light polypeptide kinase; smooth muscle myosin light chain kinase; MYLK elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human MYLK. No significant cross-reactivity or interference between Human MYLK and analogues was observed.
Sequence Length
1914
Samples
Serum, plasma, tissue homogenates, Cell lysates
Detection Range
0.78 ng/ml-50 ng/ml
Sensitivity
0.195 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MYLK elisa kit
Introduction: Myosin-light-chain kinase also known as MYLK or MLCK is a serine/threonine-specific protein kinase that phosphorylates the regulatory light chain of myosin II. These enzymes are important in the mechanism of contraction in muscle. Once there is an influx of calcium cations (Ca++) into the muscle, either from the sarcoplasmic reticulum or, more importantly, from the extracellular space, contraction of smooth muscle fibers may begin. First, the calcium will bind to calmodulin. This binding will activate MLCK, which will go on to phosphorylate the myosin light chain at serine residue 19. This will enable the myosin crossbridge to bind to the actin filament and allow contraction to begin (through the crossbridge cycle). Since smooth muscle does not contain a troponin complex like striated muscle does, this mechanism is the main pathway for regulating smooth muscle contraction.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MLCK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MLCK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MLCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MLCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MLCK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MLCK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MLCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MLCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
210,715 Da
NCBI Official Full Name
myosin light chain kinase, smooth muscle isoform 1
NCBI Official Synonym Full Names
myosin light chain kinase
NCBI Official Symbol
MYLK
NCBI Official Synonym Symbols
KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
NCBI Protein Information
myosin light chain kinase, smooth muscle; telokin; kinase-related protein; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase
UniProt Protein Name
Myosin light chain kinase, smooth muscle
UniProt Gene Name
MYLK
UniProt Synonym Gene Names
MLCK; MLCK1; MYLK1; MLCK; smMLCK; KRP
UniProt Entry Name
MYLK_HUMAN
Similar Products
Product Notes
The Human MYLK mylk (Catalog #AAA80532) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA80532 ELISA Kit recognizes Human MYLK. It is sometimes possible for the material contained within the vial of "myosin light chain kinase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.