Standard Curve (Sample)
Chicken Myogenic Differentiation antigen ELISA Kit | MyoD elisa kit
Chicken Myogenic Differentiation antigen ELISA Kit
Gene Names
MYOD1; PUM; MYF3; MYOD; bHLHc1
Reactivity
Chicken
Synonyms
Myogenic Differentiation antigen; N/A; Chicken Myogenic Differentiation antigen ELISA Kit; MyoD elisa kit
Reactivity
Chicken
Samples
Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MyoD elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This MyoD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken MyoD. This E LISA kit for research use only!
PRINCIPLE OF THE ASSAY MyoD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MyoD antibody and an MyoD-HRP conjugate. The assay sample and buffer are incubated together with MyoD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MyoD concentration since MyoD from samples and MyoD-HRP conjugate compete for the anti-MyoD antibody binding site. Since the number of sites is limited, as more sites are occupied by MyoD from the sample, fewer sites are left to bind MyoD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MyoD concentration in each sample is interpolated from this standard curve.
INTENDED USE This MyoD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken MyoD. This E LISA kit for research use only!
PRINCIPLE OF THE ASSAY MyoD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MyoD antibody and an MyoD-HRP conjugate. The assay sample and buffer are incubated together with MyoD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MyoD concentration since MyoD from samples and MyoD-HRP conjugate compete for the anti-MyoD antibody binding site. Since the number of sites is limited, as more sites are occupied by MyoD from the sample, fewer sites are left to bind MyoD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MyoD concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MyoD elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,501 Da
NCBI Official Full Name
MyoD
NCBI Official Synonym Full Names
myogenic differentiation 1
NCBI Official Symbol
MYOD1
NCBI Official Synonym Symbols
PUM; MYF3; MYOD; bHLHc1
NCBI Protein Information
myoblast determination protein 1; myf-3; myogenic factor 3; class C basic helix-loop-helix protein 1
UniProt Protein Name
Myoblast determination protein 1
UniProt Gene Name
MYOD1
UniProt Synonym Gene Names
BHLHC1; MYF3; MYOD; bHLHc1; Myf-3
UniProt Entry Name
MYOD1_HUMAN
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Product Notes
The Chicken MyoD myod1 (Catalog #AAA91605) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA91605 ELISA Kit recognizes Chicken MyoD. It is sometimes possible for the material contained within the vial of "Myogenic Differentiation antigen, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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