Standard Curve (Sample)
Rat N-Acetylgalactosaminidase Alpha ELISA Kit | NAGa elisa kit
Rat N-Acetylgalactosaminidase Alpha (NAGa) (Sandwich ELISA) ELISA Kit
Reactivity
Rat
Synonyms
N-Acetylgalactosaminidase Alpha; N/A; Rat N-Acetylgalactosaminidase Alpha (NAGa) (Sandwich ELISA) ELISA Kit; Alpha-N-Acetylgalactosaminidase; Alpha-galactosidase B; ELISA Kit for N-Acetylgalactosaminidase Alpha (NAGa); NAGa elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of NAGa. No significant cross-reactivity or interference between NAGa and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.057ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NAGa were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NAGa were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
4 degree C for 1 month/ -20 degree C for long-term(within shelf life). Shelf life is one year
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NAGa elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to NAGa. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NAGa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NAGa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NAGa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NAGa in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NAGa in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Product Categories/Family for NAGa elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,871 Da
NCBI Official Full Name
alpha-N-acetylgalactosaminidase
NCBI Official Synonym Full Names
N-acetyl galactosaminidase, alpha
NCBI Official Symbol
Naga
NCBI Protein Information
alpha-N-acetylgalactosaminidase; alpha-galactosidase B
UniProt Protein Name
Alpha-N-acetylgalactosaminidase
UniProt Gene Name
Naga
UniProt Entry Name
NAGAB_RAT
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Product Notes
The Rat NAGa naga (Catalog #AAA189927) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA189927 ELISA Kit recognizes Rat NAGa. It is sometimes possible for the material contained within the vial of "N-Acetylgalactosaminidase Alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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