Sequence
HIV-1 gp140 recombinant protein
HIV-1 gp140 (Clade C) protein (amino acid 24-680), trimer majority
Applications
ELISA
Synonyms
HIV-1 gp140; N/A; HIV-1 gp140 (Clade C) protein (amino acid 24-680), trimer majority; gp140 (HIV-1/Clade C); gp140 (HIV-1/Clade C) (aa 24-680), glycosylated recombinant protein; HIV-1 gp140 recombinant protein
Host
293 cell culture
Specificity
> 95% purity (SDS PAGE).
Concentration
1 mg/ml in PBS. (varies by lot)
Sequence Length
9041
Applicable Applications for HIV-1 gp140 recombinant protein
Western blot standard, antibody ELISA, HIV-1 entry inhibition, etc.
Viral Protein
C-terminal 6xHis tagged HIV-1 gp140 (Clade C) (isolate C.ZA.1197MB) protein (amino acid 24-680) (GenBank No. AY463234). Majority of protein exists as trimer.
Preparation and Storage
Store at -20 °C; Stable for 6-months from the date of shipment when kept at 4 °C. Non-hazardous. No MSDS required.
Sequence
Sequence
SDS-PAGE
(SDS-PAGE: Purified HIV-1 gp140 (Clade C) protein)
SDS-PAGE
(SDS-PAGE: Purified HIV-1 gp140 (Clade C) protein)
Related Product Information for HIV-1 gp140 recombinant protein
The exposed trimeric envelope glycoproteins of HIV target viral entry by melding the membranes of host cells with the virus. It consists of a structure of heterodimers of the transmembrane glycoprotein (gp41) and a surface glycoprotein (gp120) which are both derived by proteolytic cleavage from their common gp160 precursor. The gp140 ectodomain of envelope glycoprotein is a heterodimer with mass of approximately 140 kDa, composed of the entire gp120 component, and approximately 20kDa of gp41. This ectodomain is essential for viral entry into cells as it plays a vital role in attachment to specific cell surface receptors. These receptors are DC-SIGN, Heparan Sulfate Proteoglycan and a specific interaction with the CD4 receptor, particularly on helper T-cells. Binding to CD4 induces the start of a cascade of conformational changes in GP120 and gp41 that lead to the fusion of the viral with the host cell membrane. Binding to CD4 is mainly electrostatic although there are van der Waals interactions and hydrogen bonds.
Product Categories/Family for HIV-1 gp140 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
HIV-1 isolate C.ZA.1197MB from South Africa, complete genome
UniProt Protein Name
Protein Nef
UniProt Gene Name
nef
UniProt Entry Name
Q6S7U6_9HIV1
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Product Notes
The HIV-1 gp140 nef (Catalog #AAA13754) is a Recombinant Protein produced from 293 cell culture and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HIV-1 gp140 can be used in a range of immunoassay formats including, but not limited to, Western blot standard, antibody ELISA, HIV-1 entry inhibition, etc. Researchers should empirically determine the suitability of the HIV-1 gp140 nef for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIV-1 gp140, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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