Standard Curve (Sample)
Human neurofilament, heavy polypeptide ELISA Kit | NEFH elisa kit
Human Neurofilament protein H, NF-H ELISA Kit
Gene Names
NEFH; NFH
Reactivity
Human
Synonyms
neurofilament, heavy polypeptide; N/A; Human Neurofilament protein H, NF-H ELISA Kit; Human Neurofilament protein H (NF-H) ELISA kit; NFH; neurofilament triplet H protein; neurofilament; heavy polypeptide 200kDa; heavy polypeptide; NEFH elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human NF-H. No significant cross-reactivity or interference between human NF-H and analogues was observed.
Sequence Length
1020
Samples
Serum, plasma, Cerebrospinal Fluid (CSF)
Detection Range
0.625 ng/ml - 40 ng/mL
Sensitivity
<0.156 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NEFH elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NF-H has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-H present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-H is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-H bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
112,479 Da
NCBI Official Full Name
neurofilament heavy polypeptide
NCBI Official Synonym Full Names
neurofilament, heavy polypeptide
NCBI Official Symbol
NEFH
NCBI Official Synonym Symbols
NFH
NCBI Protein Information
neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa
UniProt Protein Name
Neurofilament heavy polypeptide
UniProt Gene Name
NEFH
UniProt Synonym Gene Names
KIAA0845; NFH; NF-H
UniProt Entry Name
NFH_HUMAN
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Product Notes
The Human NEFH nefh (Catalog #AAA80866) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA80866 ELISA Kit recognizes Human NEFH. It is sometimes possible for the material contained within the vial of "neurofilament, heavy polypeptide, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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