Standard Curve (Sample)
(Typical Standard Curve for NEFL ELISA (Sandwich))
Bovine Neurofilament, Light Polypeptide ELISA Kit | NEFL elisa kit
Bovine Neurofilament, Light Polypeptide (NEFL) (Sandwich ELISA) ELISA Kit
Reactivity
Bovine
Synonyms
Neurofilament, Light Polypeptide; N/A; Bovine Neurofilament, Light Polypeptide (NEFL) (Sandwich ELISA) ELISA Kit; CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein; ELISA Kit for Neurofilament, Light Polypeptide (NEFL); NEFL elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide (NEFL).
No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) and analogues was observed. Reactive with Bovine NEFL / Neurofilament, Light Polypeptide
No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) and analogues was observed. Reactive with Bovine NEFL / Neurofilament, Light Polypeptide
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
6.3pg/mL
Detection Method
Colormetric
Assay Precision
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Preparation and Storage
4 degree C for 1 month/ -20 degree C for long-term(within shelf life). Shelf life is one year
Standard Curve (Sample)
(Typical Standard Curve for NEFL ELISA (Sandwich))
Standard Curve (Sample)
(Typical Standard Curve for NEFL ELISA (Sandwich))
Related Product Information for NEFL elisa kit
Principle of the Assay||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofilament, Light Polypeptide (NEFL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofilament, Light Polypeptide (NEFL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofilament, Light Polypeptide (NEFL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurofilament, Light Polypeptide (NEFL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for NEFL elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,646 Da
NCBI Official Full Name
neurofilament light polypeptide
NCBI Official Synonym Full Names
neurofilament, light polypeptide
NCBI Official Symbol
NEFL
NCBI Protein Information
neurofilament light polypeptide; 68 kDa neurofilament protein; NF-L; micro glutamic acid-rich protein; neurofilament triplet L protein; neurofilament, light polypeptide 68kDa
UniProt Protein Name
Neurofilament light polypeptide
UniProt Gene Name
NEFL
UniProt Synonym Gene Names
NF-L
UniProt Entry Name
NFL_BOVIN
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Product Notes
The Bovine NEFL nefl (Catalog #AAA190085) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA190085 ELISA Kit recognizes Bovine NEFL. It is sometimes possible for the material contained within the vial of "Neurofilament, Light Polypeptide, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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