Human neurofilament, light polypeptide ELISA Kit | NEFL elisa kit

Human Neurofilament protein L, NF-L ELISA Kit

Cat. Num. AAA15778

• Gene Names: NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
• Reactivity: Human
• Synonyms: neurofilament; light polypeptide; Human Neurofilament protein L; NF-L ELISA Kit; Human Neurofilament protein L (NF-L) ELISA kit; CMT1F; CMT2E; FLJ53642; NF-L; NF68; NFL; light molecular weight neurofilament protein; neurofilament protein; light chain; neurofilament subunit NF-L; neurofilament triplet L protein; lig; NEFL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human NF-L. No significant cross-reactivity or interference between human NF-L and analogues was observed.
• Sequence Length: 543
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma, tissue homogenates
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: < 7.8 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for NEFL elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NF-L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-L is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-L bound in the initial step. The color development is stopped and the intensity of the color is measured.

References

Cerebrospinal fluid ceramides from patients with multiple sclerosis impair neuronal bioenergetics. Oscar G. Vidaurre, Jeffery D. Haines, Ilana Katz Sand, Kadidia P. Adula, Jimmy L. Huynh, Corey A. McGraw, Fan Zhang, Merina Varghese, Elias Sotirchos, Pavan Bhargava, Veera Venkata Ratnam Bandaru, Giulio Pasinetti, Weijia Zhang, Matilde Inglese, Peter A. Calabresi, Gang Wu, Aaron E. Miller, Norman J. Haughey, Fred D. Lublin, and Patrizia Casaccia (2014)

NCBI and Uniprot Product Information

• NCBI GI #: 105990539
• NCBI GeneID: 4747
• NCBI Accession #: NP_006149.2
• NCBI GenBank Nucleotide #: NM_006158.4
• UniProt Accession #: P07196; Q16154; Q8IU72; B9ZVN2
• Molecular Weight: 61,517 Da
• NCBI Official Full Name: neurofilament light polypeptide
• NCBI Official Synonym Full Names: neurofilament, light polypeptide
• NCBI Official Symbol: NEFL
• NCBI Official Synonym Symbols: NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
• NCBI Protein Information: neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein; protein phosphatase 1, regulatory subunit 110
• UniProt Protein Name: Neurofilament light polypeptide
• UniProt Gene Name: NEFL
• UniProt Synonym Gene Names: NF68; NFL; NF-L
• UniProt Entry Name: NFL_HUMAN