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product-image-AAA22138_SDS_PAGE.jpg SDS-PAGE

NEO recombinant protein

Recombinant Klebsiella Pneumoniae NEO Protein

Purity
>95% as determined by reducing SDS-PAGE.
Synonyms
NEO; N/A; Recombinant Klebsiella Pneumoniae NEO Protein; Aminoglycoside 3'-phosphotransferase; APH(3')-II; APH(3')II; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo; NEO recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
>95% as determined by reducing SDS-PAGE.
Form/Format
Supplied as a 0.2 um filtered solution of 20mM Tris-HCI, 6% Trehalose, 4% Mannitol, 0.05% Tween80. pH 8.0
Concentration
1.28 mg/mL (varies by lot)
Sequence Length
264
Species
Klebsiella Pneumoniae
Sequence
Met1-Phe264
Endotoxin
< 1.0 EU per ug as determined by the LAL method.
Preparation and Storage
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice. Upon receipt, store it immediately at <-20°C.

SDS-PAGE

product-image-AAA22138_SDS_PAGE.jpg SDS-PAGE
Related Product Information for NEO recombinant protein
Background: Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria.

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
Molecular Mass: 29kDa
Actual Protein Molecular Mass: 26-30kDa
NCBI Official Full Name
Aminoglycoside 3'-phosphotransferase

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Product Notes

The NEO (Catalog #AAA22138) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NEO, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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