Standard Curve (Sample)
Human Nuclear factor NF kappa B p105 subunit ELISA Kit | NFKB1/p105 elisa kit
Human Nuclear factor NF kappa B p105 subunit ELISA Kit
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Human
Synonyms
Nuclear factor NF kappa B p105 subunit; N/A; Human Nuclear factor NF kappa B p105 subunit ELISA Kit; NFKB1/p105 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NFKB1/p105 elisa kit
Principle of the assay: NFKB1/p105 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NFKB1/p105 antibody and an NFKB1/p105-HRP conjugate. The assay sample and buffer are incubated together with NFKB1/p105-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFKB1/p105 concentration since NFKB1/p105 from samples and NFKB1/p105-HRP conjugate compete for the anti-NFKB1/p105 antibody binding site. Since the number of sites is limited, as more sites are occupied by NFKB1/p105 from the sample, fewer sites are left to bind NFKB1/p105-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFKB1/p105 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,520 Da
NCBI Official Full Name
nuclear factor NF-kappa-B p105 subunit isoform 1
NCBI Official Synonym Full Names
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
NCBI Official Symbol
NFKB1
NCBI Official Synonym Symbols
p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
NCBI Protein Information
nuclear factor NF-kappa-B p105 subunit; NF-kappabeta; DNA binding factor KBF1; DNA-binding factor KBF1; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA binding subunit
UniProt Protein Name
Nuclear factor NF-kappa-B p105 subunit
UniProt Gene Name
NFKB1
UniProt Entry Name
NFKB1_HUMAN
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Product Notes
The Human NFKB1/p105 nfkb1 (Catalog #AAA96781) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA96781 ELISA Kit recognizes Human NFKB1/p105. It is sometimes possible for the material contained within the vial of "Nuclear factor NF kappa B p105 subunit, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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