Rat nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA Kit | NFKB1 elisa kit

Rat Nuclear factor-kappa B, NF- degree B ELISA Kit

Cat. Num. AAA80311

• Gene Names: Nfkb1; EBP-1; NF-kB
• Reactivity: Rat
• Synonyms: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; N/A; Rat Nuclear factor-kappa B, NF- degree B ELISA Kit; Rat Nuclear factor-kappa B (NF-kappaB) ELISA Kit; DKFZp686C01211; EBP-1; KBF1; MGC54151; NF-kappa-B; NF-kappaB; NFKB-p105; NFKB-p50; p105; p50; DNA binding factor KBF1; NF-kappabeta; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA bi; NFKB1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat NF-kB. No significant cross-reactivity or interference between rat NF-kB and analogues was observed.
• Sequence Length: 522
• Samples: Serum, plasma and tissue homogenates
• Assay Type: Sandwich
• Detection Range: 1.56 pg/ml -100 pg/ml.
• Sensitivity: The minimum detectable dose of rat NF-kB is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for NFKB1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NF-kappaB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-kappaB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-kappaB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-kappaB bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 2500134
• NCBI GeneID: 81736
• UniProt Accession #: Q63369
• Molecular Weight: 56,554 Da
• NCBI Official Full Name: Nuclear factor NF-kappa-B p105 subunit
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• NCBI Official Symbol: Nfkb1
• NCBI Official Synonym Symbols: EBP-1; NF-kB
• NCBI Protein Information: nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; nuclear factor of kappa light chain gene enhancer in B-cells 1, p105; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, p105
• UniProt Protein Name: Nuclear factor NF-kappa-B p105 subunit
• UniProt Gene Name: Nfkb1
• UniProt Entry Name: NFKB1_RAT