Standard Curve (Sample)
Human Cystatin C ELISA Kit | NGAL elisa kit
Cystatin C: Human, rapid (ELISA)
Gene Names
CST3; ARMD11; HEL-S-2
Reactivity
Human
Synonyms
Cystatin C; N/A; Cystatin C: Human, rapid (ELISA); Rapid human cystatin C; NGAL elisa kit
Reactivity
Human
Specificity
The antibodies used in this assay are specific to human cystatin c and do not cross-react with mouse and rat cystatin c, and other cytokine or hormone molecules.
Sequence Length
146
Samples
Serum, plasma and urine samples
Assay Type
Quantitative Sandwich
Sensitivity
0.23 ng/ml
Intra-assay Precision
Three samples of known concentration were tested 12 times on one plate.
Inter-assay Precision
Three samples of known concentration were tested in 10 separate assays.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NGAL elisa kit
Intended Uses: This Rapid Human Cystatin C ELISA kit is designed for quantification of human cystatin c in serum, plasma and urine samples.
Principle of the Assay: This assay is a sandwich ELISA designed for the quantitative detection of human cystatin c in samples in 1 hour. The immunoplate is pre-coated with antibody specific to human cystatin c. Standards and samples are pipetted into the wells and any human cystatin c present is sandwiched by the immobilised antibody and a second horseradish peroxidase (HRP)-linked antibody specific to human cystatin c that is co-incubated with the samples. After wash step to remove any unbound substances, the HRP substrate solution is added and colour develops in proportion to the amount of human cystatin c bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured human cystatin c, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Background/Introduction: Human cystatin c (or cystatin 3), which is composed of 120 amino acid residues, belongs to the cystatins superfamilly that inactivates lysosomal cysteine proteinases. As a strongly cationic and low-molecular weight (13.4 kDa) protein, it is almost freely filtered across the glomerular membrane, and is mainly used as a biomarker of kidney function. A growing body of evidence suggests that cystatin c is a more reliable biomarker of glomerular filtration rate than creatinine [1-3]. In addition to kidney disease, altered serum levels of cystatin c are associated with several types of cardiovascular disease, including myocardial infarction, stroke, heart failure, peripheral arterial disease and metabolic syndrome [4-7]. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer's disease [8, 9]. Furthermore, Cystatin c has also been investigated as a prognostic marker in several forms of cancer [11, 12].
Principle of the Assay: This assay is a sandwich ELISA designed for the quantitative detection of human cystatin c in samples in 1 hour. The immunoplate is pre-coated with antibody specific to human cystatin c. Standards and samples are pipetted into the wells and any human cystatin c present is sandwiched by the immobilised antibody and a second horseradish peroxidase (HRP)-linked antibody specific to human cystatin c that is co-incubated with the samples. After wash step to remove any unbound substances, the HRP substrate solution is added and colour develops in proportion to the amount of human cystatin c bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured human cystatin c, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Background/Introduction: Human cystatin c (or cystatin 3), which is composed of 120 amino acid residues, belongs to the cystatins superfamilly that inactivates lysosomal cysteine proteinases. As a strongly cationic and low-molecular weight (13.4 kDa) protein, it is almost freely filtered across the glomerular membrane, and is mainly used as a biomarker of kidney function. A growing body of evidence suggests that cystatin c is a more reliable biomarker of glomerular filtration rate than creatinine [1-3]. In addition to kidney disease, altered serum levels of cystatin c are associated with several types of cardiovascular disease, including myocardial infarction, stroke, heart failure, peripheral arterial disease and metabolic syndrome [4-7]. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer's disease [8, 9]. Furthermore, Cystatin c has also been investigated as a prognostic marker in several forms of cancer [11, 12].
References
1.Cystatin C: an emerging biomarker in cardiovascular disease. Angelidis C, et al. Curr Top Med Chem. 2013;13(2):164-79.
2. Update on cystatin C: incorporation into clinical practice. Shlipak MG, Mattes MD, Peralta CA. Am J Kidney Dis. 2013 62(3):595-603.
2. Update on cystatin C: incorporation into clinical practice. Shlipak MG, Mattes MD, Peralta CA. Am J Kidney Dis. 2013 62(3):595-603.
NCBI and Uniprot Product Information
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Product Notes
The Human NGAL (Catalog #AAA60545) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA60545 ELISA Kit recognizes Human NGAL. It is sometimes possible for the material contained within the vial of "Cystatin C, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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