NLRP3 recombinant protein

Human NLRP3 Protein, DYKDDDDK Tag

Cat. Num. AAA47508

• Gene Names: NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
• Purity: > 85% as determined by SDS-PAGE and Coomassie blue staining.
• Synonyms: NLRP3; N/A; Human NLRP3 Protein, DYKDDDDK Tag; AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NALP3; PYPAF1; NLRP3 recombinant protein

• Host: HEK293
• Purity/Purification: > 85% as determined by SDS-PAGE and Coomassie blue staining.
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose is added as protectants before lyophilization.
• Concentration: 1.92 mg/mL (varies by lot)

• Molecular Characterization: NLRP3 (Lys2-Trp1036) ; DYKDDDDK
• Tag: C-DYKDDDDK Tag
• Reconstitution: 1) Before opening the tube cap , centrifuge the sample tube at 5000g for 3-5 minutes at room temperature to ensure the lyophilized sample to settle down at the bottom of the tube.; 2) Dissolved lyophilized protein in sterile and low endotoxin water based on the recommended volume 26.04ul.; 3)After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigoroulsy pipette sample.
• Visual Inspection: Test: Lyophilized ; Specification: Dried white powder ; Result: Pass; ; Test: Vial Label ; Specification: Label aligned and complete ; Result: Pass; ; Test: Bottle Sealing (manual check) ; Specification: Sealed tight ; Result: Pass
• Quantity: Test: Bradford method ; Specification: > specified quantity ; Result: Pass
• Other Properties: Test: pH ; Specification: 7.4 ± 0.5 ; Result: Pass
• Preparation and Storage: Store at -20 degree C to -80 degree C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80 degree C (Avoid repeated freezing and thawing). ; Shipping:; The product shipped at -4 degree C. Upon receipt, store it immediately at -20 degree C or lower for long term storage.

SDS-PAGE

(Figure 1. Human NLRP3 Protein, DYKDDDDK Tag on SDS-PAGE under reducing condition.)

Related Product Information for NLRP3 recombinant protein

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 34878690
• NCBI GeneID: 114548
• NCBI Accession #: NP_899632
• NCBI GenBank Nucleotide #: NM_183395.2
• UniProt Accession #: Q96P20; O75434; Q17RS2; Q59H68; Q5JQS8; Q5JQS9; Q6TG35; Q8TCW0; B2RC97; B7ZKS9; B7ZKT2; B7ZKT3
• Molecular Weight: The protein has a predicted molecular mass of 121.8 kDa after removal of the signal peptide .
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 3 isoform b
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 3
• NCBI Official Symbol: NLRP3
• NCBI Official Synonym Symbols: AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 3; cryopyrin; caterpiller protein 1.1; PYRIN-containing APAF1-like protein 1; NACHT, LRR and PYD containing protein 3; cold autoinflammatory syndrome 1 protein; NACHT domain-, leucine-rich repeat-, and PYD-con
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 3
• UniProt Gene Name: NLRP3
• UniProt Synonym Gene Names: C1orf7; CIAS1; NALP3; PYPAF1; CLR1.1
• UniProt Entry Name: NALP3_HUMAN