Influenza A virus Nucleoprotein (NP) Recombinant Protein | NP recombinant protein

Recombinant Influenza A virus Nucleoprotein (NP)

Cat. Num. AAA18706

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Influenza A virus Nucleoprotein (NP); N/A; Recombinant Influenza A virus Nucleoprotein (NP); Nucleocapsid protein; Protein N; NP recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-498aa; Full Length Protein
• Sequence: MASQGTKRSYEQMETDGERQNATEIRASVGKMIGGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSAFDERRNKYLEEHPSAGKDPKKTGGPIYRRVNGKWMRELILYDKEEIRRIWRQANNGDDATAGLTHMMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELVRMIKRGINDRNFWRGENGRKTRIAYERMCNILKGKFQTAAQKAMMDQVRESRNPGNAEFEDLTFLARSALILRGSVAHKSCLPACVYGPAVASGYDFEREGYSLVGIDPFRLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRVLSFIKGTKVLPRGKLSTRGVQIASNENMETMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISIQPTFSVQRNLPFDRTTIMAAFNGNTEGRTSDMRTEIIRMMESARPEDVSFQGRGVFELSDEKAASPIVPSFDMSNEGSYFFGDNAEEYDN

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for NP recombinant protein

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP ses to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.

Product Categories/Family for NP recombinant protein

Microbiology

References

The structure of the gene encoding the nucleoprotein of human influenza virus A/PR/8/34.Winter G., Fields S.Virology 114:423-428(1981) Complete nucleotide sequence of the nucleoprotein gene from the human influenza strain A/PR/8/34 (HON1) .van Rompuy L., Min Jou W., Huylebroeck D., Devos R., Fiers W.Eur. J. Biochem. 116:347-353(1981) Erratumvan Rompuy L., Min Jou W., Huylebroeck D., Devos R., Fiers W.Eur. J. Biochem. 116:645-645(1981) Plasmid-only rescue of influenza A virus vaccine candidates.Schickli J.H., Flandorfer A., Nakaya T., Martinez-Sobrido L., Garcia-Sastre A., Palese P.Philos. Trans. R. Soc. Lond., B, Biol. Sci. 356:1965-1973(2001) Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments.de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.Virus Res. 103:155-161(2004) Fluorescent antigen-transfected target cell cytotoxic T lymphocyte assay for ex vivo detection of antigen-specific cell-mediated cytotoxicity.van Baalen C.A., Kwa D., Verschuren E.J., Reedijk M.L., Boon A.C., de Mutsert G., Rimmelzwaan G.F., Osterhaus A.D., Gruters R.A.J. Infect. Dis. 192:1183-1190(2005) The NIAID influenza genome sequencing project.Ghedin E., Spiro D., Miller N., Zaborsky J., Feldblyum T., Subbu V., Shumway M., Sparenborg J., Groveman L., Halpin R., Sitz J., Koo H., Salzberg S.L., Webster R.G., Hoffmann E., Krauss S., Naeve C., Bao Y., Bolotov P., Dernovoy D., Kiryutin B., Lipman D.J., Tatusova T.A classical bipartite nuclear localization signal on Thogoto and influenza A virus nucleoproteins.Weber F., Kochs G., Gruber S., Haller O.Virology 250:9-18(1998) Modulation of nuclear localization of the influenza virus nucleoprotein through interaction with actin filaments.Digard P., Elton D., Bishop K., Medcalf E., Weeds A., Pope B.J. Virol. 73:2222-2231(1999) Oligomerization of the influenza virus nucleoprotein identification of positive and negative sequence elements.Elton D., Medcalf E., Bishop K., Digard P.Virology 260:190-200(1999) Identification of amino acid residues of influenza virus nucleoprotein essential for RNA binding.Elton D., Medcalf L., Bishop K., Harrison D., Digard P.J. Virol. 73:7357-7367(1999) Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway.Elton D., Simpson-Holley M., Archer K., Medcalf L., Hallam R., McCauley J., Digard P.J. Virol. 75:408-419(2001)

NCBI and Uniprot Product Information

• NCBI GI #: 8486130
• NCBI GeneID: 956531
• NCBI Accession #: NP_040982.1
• NCBI GenBank Nucleotide #: NC_002019.1
• UniProt Accession #: P03466; Q20N34; Q58NB3; Q67228; Q80AB4; AAA43467
• Molecular Weight: 58.2 kDa
• NCBI Official Full Name: nucleocapsid protein
• NCBI Official Symbol: NP
• NCBI Protein Information: nucleocapsid protein
• UniProt Protein Name: Nucleoprotein
• UniProt Gene Name: NP
• UniProt Synonym Gene Names: Protein N
• UniProt Entry Name: NCAP_I34A1