NRAS recombinant protein

Recombinant Human NRAS Protein (His tag)

Cat. Num. AAA173537

• Gene Names: NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• Purity: > 95 % as determined by reducing SDS-PAGE
• Synonyms: NRAS; N/A; Recombinant Human NRAS Protein (His tag); NRAS, HRAS1; NRAS recombinant protein

• Host: E Coli
• Purity/Purification: > 95 % as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 100mm NACI, 10% Glycerol, 5% Trehalose, 5% Mannitol, 0.01% Tween-80
• Sequence: Met1- Cys186
• Sequence Length: 189

• Species: Human
• Tag: N-His
• Reconstitution: MBS recommends that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that the sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL . Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized protein should be stored at < -20°C, Reconstituted protein can be stored at 4-7°C for 2-7 days. Aliquots of reconsituted samples are stable at < -20°C for 3 months.

SDS-PAGE

Related Product Information for NRAS recombinant protein

Background: NRAS was discovered by researchers at the Institute of Cancer Research, funded by the Cancer Research Campaign (now Cancer Research UK). NRAS gene is a member of the Ras gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The mammalian ras gene family consists of the harvey and kirsten ras genes (HRAS and KRAS), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. The NRAS gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The NRAS gene consists of seven exons (-I, I, II, III, IV, V, VI)

NCBI and Uniprot Product Information

• NCBI GI #: 4505451
• NCBI GeneID: 4893
• NCBI Accession #: NP_002515.1
• NCBI GenBank Nucleotide #: NM_002524.4
• UniProt Accession #: P01111; Q14971; Q15104; Q15282
• Molecular Weight: Mol_Mass: 23kDa; AP_Mol_Mass: 24kDa
• NCBI Official Full Name: GTPase NRas
• NCBI Official Synonym Full Names: neuroblastoma RAS viral oncogene homolog
• NCBI Official Symbol: NRAS
• NCBI Official Synonym Symbols: NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• NCBI Protein Information: GTPase NRas
• UniProt Protein Name: GTPase NRas
• UniProt Gene Name: NRAS
• UniProt Synonym Gene Names: HRAS1