Rat Neuropilin-1 ELISA Kit | NRP1 elisa kit

Rat Neuropilin-1 PicoKine ELISA Kit

Cat. Num. AAA45867

• Gene Names: Nrp1; Nrp
• Reactivity: Rat
• Synonyms: Neuropilin-1; N/A; Rat Neuropilin-1 PicoKine ELISA Kit; neuropilin 1; A5 protein; BDCA4; BLOOD DENDRITIC CELL ANTIGEN 4; CD 304; CD304; DKFZp686A03134; DKFZp781F1414; Neuropilin1; Neuropilin1 transmembrane receptor; NP1; NPN1; NRP 1; NRP; NRP1; NRP1_HUMAN; Vascular endothelial cell growth factor 165 receptor; VEGF165R; NRP1 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Neuropilin-1
• Sequence Length: 922
• Samples: Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
• Assay Type: Sandwich
• Detection Range: 780pg/ml-50000pg/ml
• Sensitivity: <20pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Rat Neuropilin-1 PicoKine ELISA Kit standard curve)

Related Product Information for NRP1 elisa kit

Principle of the Assay: Rat Neuropilin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neuropilin-1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: F22-D854) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuropilin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Neuropilin-1 amount of sample captured in plate.

Background: NRP1(Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.

NCBI and Uniprot Product Information

• NCBI GI #: 9297002
• NCBI GeneID: 246331
• UniProt Accession #: Q9QWJ9
• Molecular Weight: 103,082 Da
• NCBI Official Full Name: Neuropilin-1
• NCBI Official Synonym Full Names: neuropilin 1
• NCBI Official Symbol: Nrp1
• NCBI Official Synonym Symbols: Nrp
• NCBI Protein Information: neuropilin-1; vascular endothelial cell growth factor 165 receptor
• UniProt Protein Name: Neuropilin-1
• UniProt Gene Name: Nrp1
• UniProt Entry Name: NRP1_RAT