NSE recombinant protein
NSE Protein (Active)
Applications
SDS-Page, ELISA
Purity
>95% by SDS-PAGE
Synonyms
NSE; N/A; NSE Protein (Active); Neuron Specific Enolase; NSE recombinant protein
Host
E Coli
Purity/Purification
>95% by SDS-PAGE
Form/Format
Lyophilized
Applicable Applications for NSE recombinant protein
SDS-PAGE, ELISA
Storage Buffer
Each vial contains 10 mM Tris-HCl (pH8.0 ± 0.2) with 0.05% NaN3.
Preparation and Storage
Upon receipt, aliquot and store at -20°C or -80°C for long term. Avoid repeated freeze and thaw cycles
Related Product Information for NSE recombinant protein
Recombinant full length human NSE protein with molecular weight 56 kDa.
The enolases (phosphopyruvate hydratase) catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. The functional enzyme is a dimer made up of subunits referred to as alpha, beta, and gamma. Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. ENO2 is the major form found in mature neurons and in cells of neuronal origin. The homozygous deletions in passenger genes in cancer deletions can expose cancer-specific therapeutic vulnerabilities when the collaterally deleted gene is a member of a functionally redundant family of genes carrying out an essential function. The glycolytic gene ENO1 in the 1p36 locus is deleted in glioblastoma, which is tolerated by the expression of ENO2. The short-hairpin-RNA-mediated silencing of ENO2 selectively inhibits growth, survival and the tumorigenic potential of ENO1-deleted GBM cells, and that the enolase inhibitor phosphonoacetohydroxamate is selectively toxic to ENO1-deleted GBM cells relative to ENO1-intact GBM cells or normal astrocytes.
The enolases (phosphopyruvate hydratase) catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. The functional enzyme is a dimer made up of subunits referred to as alpha, beta, and gamma. Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. ENO2 is the major form found in mature neurons and in cells of neuronal origin. The homozygous deletions in passenger genes in cancer deletions can expose cancer-specific therapeutic vulnerabilities when the collaterally deleted gene is a member of a functionally redundant family of genes carrying out an essential function. The glycolytic gene ENO1 in the 1p36 locus is deleted in glioblastoma, which is tolerated by the expression of ENO2. The short-hairpin-RNA-mediated silencing of ENO2 selectively inhibits growth, survival and the tumorigenic potential of ENO1-deleted GBM cells, and that the enolase inhibitor phosphonoacetohydroxamate is selectively toxic to ENO1-deleted GBM cells relative to ENO1-intact GBM cells or normal astrocytes.
References
1. Muller, F. L., Colla, S., Aquilanti, E., Manzo, V. E., Genovese, G., Lee, J., Eisenson, D., Narurkar, R., Deng, P., Nezi, L., Lee, M. A., Hu, B., and 10 others. Passenger deletions generate therapeutic vulnerabilities in cancer. Nature 488: 337-342, 2012.
Similar Products
Product Notes
The NSE (Catalog #AAA59093) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NSE can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, ELISA. Researchers should empirically determine the suitability of the NSE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSE, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.