SDS-PAGE
NT5C2 recombinant protein
NT5C2, 1-561aa, Human, His tag E Coli
Gene Names
NT5C2; GMP; NT5B; PNT5; SPG45; SPG65; cN-II
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
NT5C2; N/A; NT5C2, 1-561aa, Human, His tag E Coli; Cytosolic purine 5'-nucleotidase; cN-II; GMP. NT5B; PNT5; NT5C2 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E
Sequence Length
561
Applicable Applications for NT5C2 recombinant protein
SDS-PAGE
Preparation and Storage
Can be stored at 4°C short term (1-2 weeks).
For long term storage, aliquot and store at -20°C or -70°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C or -70°C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for NT5C2 recombinant protein
NT5C2, also known as NT5B or PNT5, has an essential role in the maintenance of purine/pyrimidine nucleotides. It contains a phosphotransferase active site that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates. In addition, it regulates the level of inosine monophosphate (IMP) and guanosine monophosphate (GMP) pools within cells via hydrolysis. Recombinant human NT5C2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for NT5C2 recombinant protein
References
Oka J. et al. (1994) Biochem. Biophys. Res. Commun. 205: 917-922.; Wallde K. et al. (2007) J Biol Chem. 282: 17828-17836.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67.1 kDa (581aa)
NCBI Official Full Name
cytosolic purine 5'-nucleotidase isoform 1
NCBI Official Synonym Full Names
5'-nucleotidase, cytosolic II
NCBI Official Symbol
NT5C2
NCBI Official Synonym Symbols
GMP; NT5B; PNT5; SPG45; SPG65; cN-II
NCBI Protein Information
cytosolic purine 5'-nucleotidase
UniProt Protein Name
Cytosolic purine 5'-nucleotidase
UniProt Gene Name
NT5C2
UniProt Synonym Gene Names
NT5B; NT5CP; PNT5
Similar Products
Product Notes
The NT5C2 nt5c2 (Catalog #AAA48783) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NT5C2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the NT5C2 nt5c2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E. It is sometimes possible for the material contained within the vial of "NT5C2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.