Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse NTN1. No significant cross-reactivity or interference between Mouse NTN1 and analogues was observed.
Sequence Length
604
Samples
Serum, plasma, tissue homogenates
Assay Type
Competitive
Detection Range
3.12 pg/ml-200 pg/ml
Sensitivity
0.78 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Apoptosis/Autophagy
Protein Biological Process 3
Apoptosis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for NTN1 elisa kit
Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. An antibody specific for NTN1 has been pre-coated onto a microplate. Add NTN1 (Standards or samples) to the well, and then add Biotin-conjugated NTN1. A competitive inhibition reaction is launched between NTN1 (Standards or samples) and Biotin conjugated NTN1 with the NTN1 antibody. Then add the HRP-avidin to each well. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of NTN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,810 Da
NCBI Official Full Name
netrin-1
NCBI Official Synonym Full Names
netrin 1
NCBI Official Symbol
Ntn1
NCBI Official Synonym Symbols
AI561871; BC019633; Netrin-1
NCBI Protein Information
netrin-1
UniProt Protein Name
Netrin-1
UniProt Gene Name
Ntn1
UniProt Entry Name
NET1_MOUSE
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Product Notes
The Mouse NTN1 ntn1 (Catalog #AAA112520) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA112520 ELISA Kit recognizes Mouse NTN1. It is sometimes possible for the material contained within the vial of "netrin 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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