Application Data
(Figure: Netrin-1 (human) (rec.) (AAA77037) mediated commissural axon outgrowth. Method: Dorsal spinal cord were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of Netrin-1 (250 ng/ml). Axons were then stained with an anti-β-tubulin antibody. Picture courtesy of Véronique Corset, Centre Léon Bérard, Lyon.)
Netrin-1 Recombinant Protein | NTN1 recombinant protein
Netrin-1 (human), (recombinant)
Gene Names
NTN1; NTN1L
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
>=90% (SDS-PAGE)Purified by multi-step chromatography
Synonyms
Netrin-1; N/A; Netrin-1 (human), (recombinant); NTN1 recombinant protein
Host
HEK293 cells
Reactivity
Human, Mouse, Rat
Specificity
Tests positive for binding to human Unc5b by ELISA. Previous lots produced from the same cell line bind to human, mouse and rat receptors UNC5H and DCC
Purity/Purification
>=90% (SDS-PAGE)
Purified by multi-step chromatography
Purified by multi-step chromatography
Form/Format
Lyophilized. Contains PBS
Concentration
0.1mg/ml after reconstitution (varies by lot)
Applicable Applications for NTN1 recombinant protein
ELISA
Source Note
The extracellular domain of human netrin-1 (aa 28-604) is fused at the N-terminus to a DYKDDDDK®-tag.
Reconstitution
Reconstitute with 100µl sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.
Scientific Background
The Netrins (whose name means “one who guides” in Sanskrit) are a family of laminin-related secreted protein. In mice, human and rat, netrin-1, netrin-3/NTL2, Netrin-4 and G-Netrins have been cloned. Netrin-1 is predominantly expressed by floor plate cells as commissural axons extend toward the ventral midline. Netrin-1 induces axonal outgrowth, axon orientation and neuronal migration in neuronal development. It is chemoattractive for some neurons and chemorepellent for others. To carry its functions, netrin-1 interacts with specific receptors: DCC/Neogenin and UNC-5. These receptors belong to the family of “dependence receptors” that induce apoptosis in the absence of their respective ligand, and promote survival when bound to their ligand. The Netrin-1 mediated survival signal inhibits p53-induced apoptosis. Netrin-1 is also expressed in organs such as pancreas, lung, bowel, bone and mammary gland. In these tissues, netrin-1 has different roles such as invasion of epithelial cells, leukocytes migration, cell adhesion or angiogenesis. Netrin-1, as a survival factor, also contributes to the control of tumorigenesis. Historical results have shown that netrin-1 induces axon outgrowth and inhibits DCC- and UNC5H2-mediated apoptosis in HEK 293 cells.
Preparation and Storage
Ships on Blue Ice
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Application Data
(Figure: Netrin-1 (human) (rec.) (AAA77037) mediated commissural axon outgrowth. Method: Dorsal spinal cord were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of Netrin-1 (250 ng/ml). Axons were then stained with an anti-β-tubulin antibody. Picture courtesy of Véronique Corset, Centre Léon Bérard, Lyon.)
Application Data
(Figure: Netrin-1 (human) (rec.) (AAA77037) mediated commissural axon outgrowth. Method: Dorsal spinal cord were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of Netrin-1 (250 ng/ml). Axons were then stained with an anti-β-tubulin antibody. Picture courtesy of Véronique Corset, Centre Léon Bérard, Lyon.)
ELISA
(ELISA analysis of Netrin-1 (human), (recombinant): Each well is coated with either 500ng Unc5b (Please inquire) or 2% BSA. Netrin-1 is titrated in 2-fold series and detected by anti-DYKDDDDK antibody.)
ELISA
(ELISA analysis of Netrin-1 (human), (recombinant): Each well is coated with either 500ng Unc5b (Please inquire) or 2% BSA. Netrin-1 is titrated in 2-fold series and detected by anti-DYKDDDDK antibody.)
SDS-PAGE
(SDS-PAGE analysis of Netrin-1 (human), (recombinant): Lane 1: MW Marker, Lane 2: 1ug Netrin-1.)
SDS-PAGE
(SDS-PAGE analysis of Netrin-1 (human), (recombinant): Lane 1: MW Marker, Lane 2: 1ug Netrin-1.)
Product Categories/Family for NTN1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~85 kDa
NCBI Official Full Name
netrin-1
NCBI Official Synonym Full Names
netrin 1
NCBI Official Symbol
NTN1
NCBI Official Synonym Symbols
NTN1L
NCBI Protein Information
netrin-1; netrin 1, mouse, homolog of; epididymis tissue protein Li 131P
UniProt Protein Name
Netrin-1
UniProt Gene Name
NTN1
UniProt Synonym Gene Names
NTN1L
UniProt Entry Name
NET1_HUMAN
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Product Notes
The NTN1 ntn1 (Catalog #AAA77037) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. The Netrin-1 (human), (recombinant) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Netrin-1 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the NTN1 ntn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Netrin-1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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