TrkA recombinant protein

Recombinant rat TrkA protein

Cat. Num. AAA48422

• Gene Names: Ntrk1; Trk
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: TrkA; N/A; Recombinant rat TrkA protein; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; NTRK-1; NTRK1; Slow nerve growth factor receptor; p140-TrkA; Trk-A; Trk; Trka; TRK1-transforming tyrosine kinase protein; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A; TrkA recombinant protein

• Host: HEK293
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
• Concentration: 1 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 35-418aa
• Sequence: SCRETCCPVG PSGLRCTRAG TLNTLRGLRG AGNLTELYVE NQRDLQRLEF EDLQGLGELR SLTIVKSGLR FVAPDAFHFT PRLSHLNLSS NALESLSWKT VQGLSLQDLT LSGNPLHCSC ALLWLQRWEQ EDLCGVYTQK LQGSGSGDQF LPLGHNNSCG VPSVKIQMPN DSVEVGDDVF LQCQVEGQAL QQADWILTEL EGTATMKKSG DLPSLGLTLV NVTSDLNKKN VTCWAENDVG RAEVSVQVSV SFPASVHLGK AVEQHHWCIP FSVDGQPAPS LRWFFNGSVL NETSFIFTQF LESALTNETM RHGCLRLNQP THVNNGNYTL LAANPYGQAA ASIMAAFMDN PFEFNPEDPI PVSFSPVDTN STSRDPVEKK DETP

• Applicable Applications for TrkA recombinant protein: Bioactivity, SDS-PAGE
• Species: Rat
• Tag: hIgG-His-Tag
• Predicted Nterm: Ser 35
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Bioactivity: Measured by ability to inhibit NGF-induced proliferation assay using TF?1 human erythroleukemic cells in the presence of 0.5ng/ml of rat NGF. The ED50 range <= 5 ng/ml.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Rat Ntrk1 in inhibit the cell growth using TF-1 human erythroleukemic cells in the presence of 0.5ng/ml of rat NGF. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for TrkA recombinant protein

TrkA, also known as Tyrosine kinase receptor A, is a member of the neurotrophic tyrosine kinase receptor(NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. It leads to cell differentiation and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as the establishment of thermal regulation via sweating. In one study conducted on two rat models, an inhibition of TrkA with AR786 led to a reduction in joint swelling, joint damage, and pain caused by inflammatory arthritis. Recombinant rat TrkA, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for TrkA recombinant protein

Neuroscience

References

Benito-Gutiérrez E, et al. (2006). Mol Cell Neurosci. 31:179-192.;; Lambiase A, et al. (2005). Natl Acad Sci. 102:16795-16800.

NCBI and Uniprot Product Information

• NCBI GI #: 11024666
• NCBI GeneID: 59109
• NCBI Accession #: NP_067600.1
• NCBI GenBank Nucleotide #: NM_021589.1
• UniProt Accession #: P35739
• Molecular Weight: 87,252 Da
• NCBI Official Full Name: high affinity nerve growth factor receptor
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 1
• NCBI Official Symbol: Ntrk1
• NCBI Official Synonym Symbols: Trk
• NCBI Protein Information: high affinity nerve growth factor receptor; trk; trk-A; p140-TrkA; TrkA neurotrophin receptor; trkA proto-oncogene receptor; tropomyosin-related kinase A; slow nerve growth factor receptor
• UniProt Protein Name: High affinity nerve growth factor receptor
• UniProt Gene Name: Ntrk1
• UniProt Synonym Gene Names: Trk; Trka; Trk-A
• UniProt Entry Name: NTRK1_RAT