Monkey Cross-linked N-terminal Telopeptides of type I collagen ELISA Kit | NTx elisa kit

Monkey Cross-linked N-terminal Telopeptides of type I collagen ELISA Kit

Cat. Num. AAA17050

• Reactivity: Monkey
• Synonyms: Cross-linked N-terminal Telopeptides of type I collagen; N/A; Monkey Cross-linked N-terminal Telopeptides of type I collagen ELISA Kit; NTx elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of NTX1. No significant cross-reactivity or interference between NTX1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NTX1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for NTx elisa kit

Principle of the Assay: NTX1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NTX1 antibody and an NTX1-HRP conjugate. The assay sample and buffer are incubated together with NTX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NTX1 concentration since NTX1 from samples and NTX1-HRP conjugate compete for the anti-NTX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NTX1 from the sample, fewer sites are left to bind NTX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NTX1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This NTX1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey NTX1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for NTx elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 4579738
• Molecular Weight: 146,743 Da
• NCBI Official Full Name: NTX, partial
• UniProt Protein Name: NTX
• UniProt Gene Name: ntx
• UniProt Entry Name: Q9QTG7_CBDP