Application Data
Ole e 1 recombinant protein
Recombinant allergen Ole e 1 for Olea europaea (olive tree)
Purity
15% SDS-PAGE
Synonyms
Ole e 1; N/A; Recombinant allergen Ole e 1 for Olea europaea (olive tree); Ole e 1 recombinant protein
Host
Pichia pastoris
Purity/Purification
15% SDS-PAGE
Form/Format
Dry powder (lyophilized)
Concentration
1.20 mg/ml (varies by lot)
Organism
Olea Europaea
Storage Buffer
20 mM phosphate buffer pH 8 and 0.15 M NaCl
Quality Control
1. Protein concentration determined spectrophotometrically.
2. Purity control in SDS-PAGE: 15%
3. No precipitate observed after a freezing and thawing cycle.
2. Purity control in SDS-PAGE: 15%
3. No precipitate observed after a freezing and thawing cycle.
Protein Fingerprint by Mass Spectrometry
Top Score: 117 for ole e 1
Protein score is -10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 13 are significant (p<0.05).
The MS was performed with a by MALDI TOF/TOF model UltrafleXtreme (Bruker).
Protein score is -10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 13 are significant (p<0.05).
The MS was performed with a by MALDI TOF/TOF model UltrafleXtreme (Bruker).
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Application Data
Application Data
SDS-PAGE
(Figure 1. SDS-PAGE analysis (15%) of 8 ul of the recombinant allergen ole e 1. Purity is >95% as determined by gel electrophoresis.)
SDS-PAGE
(Figure 1. SDS-PAGE analysis (15%) of 8 ul of the recombinant allergen ole e 1. Purity is >95% as determined by gel electrophoresis.)
Related Product Information for Ole e 1 recombinant protein
Recombinant Allergen: Olea europaea ole e 1 olive tree allergen (Asturias et al., 1997).
Description: Ole e 1 is the most prevalent allergen of olive tree pollen. This allergen has been prepared as a recombinant mature protein fused to a his-tag in its C terminus.
Description: Ole e 1 is the most prevalent allergen of olive tree pollen. This allergen has been prepared as a recombinant mature protein fused to a his-tag in its C terminus.
References
Asturias, JA et al. Cloning and expression of the panallergen profiling and the major allergen (Ole e 1) from olive tree pollen. 1997, J. Allergy Clin Immunol 100:365-72.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.
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Product Notes
The Ole e 1 (Catalog #AAA72868) is a Recombinant Protein produced from Pichia pastoris and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ole e 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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