Standard Curve (Sample)
Rat Osteonectin ELISA Kit | ON elisa kit
Rat Osteonectin ELISA Kit
Gene Names
SPARC; ON; OI17; BM-40
Reactivity
Rat
Synonyms
Osteonectin; N/A; Rat Osteonectin ELISA Kit; ON/BM-40/Basement-membrane protein 40/BM-40/ONcysteine-rich protein/osteonectin/Osteonectin/Secreted protein acidic and rich in cysteine/secreted protein; acidic; cysteine-rich (osteonectin)/; ON elisa kit
Reactivity
Rat
Sequence Length
302
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ON elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat ON antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat ON antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat ON amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat ON can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,632 Da
NCBI Official Full Name
osteonectin
NCBI Official Synonym Full Names
secreted protein acidic and cysteine rich
NCBI Official Symbol
SPARC
NCBI Official Synonym Symbols
ON; OI17; BM-40
NCBI Protein Information
SPARC
UniProt Protein Name
SPARC
UniProt Gene Name
SPARC
UniProt Synonym Gene Names
ON; BM-40; ON
UniProt Entry Name
SPRC_HUMAN
Similar Products
Product Notes
The Rat ON sparc (Catalog #AAA98708) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA98708 ELISA Kit recognizes Rat ON. It is sometimes possible for the material contained within the vial of "Osteonectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.