Osteoprotegerin/Fc Chimera Active Protein | OPG active protein

Recombinant Human Osteoprotegerin/Fc Chimera

Cat. Num. AAA10811

• Gene Names: TNFRSF11B; OPG; TR1; OCIF
• Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Osteoprotegerin/Fc Chimera; N/A; Recombinant Human Osteoprotegerin/Fc Chimera; OPG Fc Human; Osteoprotegerin Human Recombinant /Fc Chimera; TNFRSF11B; OPG; OCIF; Osteoclastogenesis inhibitory factor; TR1; MGC29565; OPG Fc; OPG active protein

• Purity/Purification: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: OPG was lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 6.0, 150mM NaCl and 0.02 % Tween-80.
• Sequence: OPG 22-201; ETFPPKYLHY DEETSHQLLC DKCPPGTYLK QHCTAKWKTV CAPCPDHYYT DSWHTSDECL YCSPVCKELQ YVKQECNRTH NRVCECKEGR YLEIEFCLKH RSCPPGFGVV QAGTPERNTV CKRCPDGFFS NETSSKAPCR KHTNCSVFGL LLTQKGNATH DNICSGNSES TQKCGIDVTL ; ; Fc232; EPKSSDKTHT CPPCPAPEFE GAPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPTPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK
• Sequence Length: 401

• Source: Pichia Pastoris
• Physical Appearance: Sterile filtered white lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized Osteoprotegerin in sterile 18M Omega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED₅₀ as determined by neutralizing the stimulation of U937 cells is less than 10ng/ml corresponding to a specific activity of > 1.0 x 10⁵ IU/mg in the presence of 10ng/ml soluble Human RANKL (sRANKL).
• Preparation and Storage: Lyophilized Osteoprotegerin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution OCIF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for OPG active protein

Description: Recombinant OPG produced in yeast contains 2x412 amino acid residues, including 180 residues from mature OPG (a.a 22-201) and 232 residues from the Fc protein of human IgG1, and has a calculated molecular mass of 109.6kDa.

Introduction: Osteoprotegerin acts as decoy receptor for rankl and thereby neutralizes its function in osteoclastogenesis. OPG inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local rankl/opg ratio. Osteoprotegerin may also play a role in preventing arterial calcification. May act as decoy receptor for trail and protect against apoptosis. Trail binding blocks the inhibition of osteoclastogenesis.

Product Categories/Family for OPG active protein

Cytokines and Growth Factors; Growth Factors; Osteoprotegerin

NCBI and Uniprot Product Information

• NCBI GI #: 148743793
• NCBI GeneID: 4982
• NCBI Accession #: NP_002537.3
• NCBI GenBank Nucleotide #: NM_002546.3
• UniProt Accession #: O00300; O60236; Q53FX6; Q9UHP4; B2R9A8
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 11B
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 11b
• NCBI Official Symbol: TNFRSF11B
• NCBI Official Synonym Symbols: OPG; TR1; OCIF
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 11B; osteoclastogenesis inhibitory factor; osteoprotegerin
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 11B
• UniProt Gene Name: TNFRSF11B
• UniProt Synonym Gene Names: OCIF; OPG
• UniProt Entry Name: TR11B_HUMAN