Rat Mu-type opioid receptor ELISA Kit | Oprm1 elisa kit

Rat Mu-type opioid receptor ELISA Kit

Cat. Num. AAA97804

• Gene Names: Oprm1; MORA; Oprm; Oprrm1
• Reactivity: Rat
• Synonyms: Mu-type opioid receptor; N/A; Rat Mu-type opioid receptor ELISA Kit; Oprm1/M-OR-1/MOR-1/Ror-b/Mu-type opioid receptor/Opioid receptor B; Oprm1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Oprm1. No significant cross-reactivity or interference between Oprm1 and analogues was observed.
• Sequence Length: 393
• Assay Type: Sandwich ELISA, Double Antibody
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oprm1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oprm1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for Oprm1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Oprm1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Oprm1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Oprm1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Oprm1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 145553980
• NCBI GeneID: 25601
• NCBI Accession #: NP_001033688.2
• NCBI GenBank Nucleotide #: NM_001038599.2
• Molecular Weight: 43,317 Da
• NCBI Official Full Name: mu-type opioid receptor isoform 1B2
• NCBI Official Synonym Full Names: opioid receptor, mu 1
• NCBI Official Symbol: Oprm1
• NCBI Official Synonym Symbols: MORA; Oprm; Oprrm1
• NCBI Protein Information: mu-type opioid receptor
• UniProt Protein Name: Mu-type opioid receptor
• UniProt Gene Name: Oprm1
• UniProt Synonym Gene Names: Ror-b; M-OR-1; MOR-1
• UniProt Entry Name: OPRM_RAT