Mouse Oncostatin M ELISA Kit | OSM elisa kit

Mouse Oncostatin M ELISA Kit

Cat. Num. AAA16449

• Reactivity: Mouse
• Synonyms: Oncostatin M; N/A; Mouse Oncostatin M ELISA Kit; OSM elisa kit

• Reactivity: Mouse
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL

• Intended Uses: This OSM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse OSM. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for OSM elisa kit

Principle of the assay: OSM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OSM antibody and an OSM-HRP conjugate. The assay sample and buffer are incubated together with OSM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OSM concentration since OSM from samples and OSM-HRP conjugate compete for the anti-OSM antibody binding site. Since the number of sites is limited, as more sites are occupied by OSM from the sample, fewer sites are left to bind OSM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OSM concentration in each sample is interpolated from this standard curve.

Product Categories/Family for OSM elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 10092621
• NCBI GeneID: 5008
• NCBI Accession #: NP_065391.1
• NCBI GenBank Nucleotide #: NM_020530.4
• Molecular Weight: 28,484 Da
• NCBI Official Full Name: oncostatin-M preproprotein
• NCBI Official Synonym Full Names: oncostatin M
• NCBI Official Symbol: OSM
• NCBI Protein Information: oncostatin-M
• UniProt Protein Name: Oncostatin-M
• UniProt Gene Name: OSM
• UniProt Synonym Gene Names: OSM
• UniProt Entry Name: ONCM_HUMAN