Mouse Phospho-Glycogen Synthase Kinase 3beta ELISA Kit | P-GSK3beta elisa kit

Mouse Phospho-Glycogen Synthase Kinase 3beta ELISA Kit

Cat. Num. AAA85319

• Gene Names: NPHS2; PDCN; SRN1
• Reactivity: Mouse
• Synonyms: Phospho-Glycogen Synthase Kinase 3beta; N/A; Mouse Phospho-Glycogen Synthase Kinase 3beta ELISA Kit; Mouse Phospho-Glycogen Synthase Kinase 3b ELISA Kit; P-GSK3beta elisa kit

• Reactivity: Mouse
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.

• Intended Uses: This PDCN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PDCN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for P-GSK3beta elisa kit

Principle of the Assay: PDCN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDCN antibody and an PDCN-HRP conjugate. The assay sample and buffer are incubated together with PDCN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDCN concentration since PDCN from samples and PDCN-HRP conjugate compete for the anti-PDCN antibody binding site. Since the number of sites is limited, as more sites are occupied by PDCN from the sample, fewer sites are left to bind PDCN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDCN concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7657615
• NCBI GeneID: 7827
• NCBI Accession #: NP_055440.1
• NCBI GenBank Nucleotide #: NM_014625.3
• Molecular Weight: 34,421 Da
• NCBI Official Full Name: podocin isoform 1
• NCBI Official Synonym Full Names: nephrosis 2, idiopathic, steroid-resistant (podocin)
• NCBI Official Symbol: NPHS2
• NCBI Official Synonym Symbols: PDCN; SRN1
• NCBI Protein Information: podocin
• UniProt Protein Name: Podocin
• UniProt Gene Name: NPHS2
• UniProt Entry Name: PODO_HUMAN