Rat Phosphotylinosital 3 kinase ELISA Kit | P13K elisa kit

Rat Phosphotylinosital 3 kinase ELISA Kit

Cat. Num. AAA84884

• Gene Names: PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA
• Reactivity: Rat
• Synonyms: Phosphotylinosital 3 kinase; N/A; Rat Phosphotylinosital 3 kinase ELISA Kit; P13K elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of PI3K. No significant cross-reactivity or interference between PI3K and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PI3K and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for P13K elisa kit

Principle of the Assay: PI3K ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PI3K antibody and an PI3K-HRP conjugate. The assay sample and buffer are incubated together with PI3K-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PI3K concentration since PI3K from samples and PI3K-HRP conjugate compete for the anti-PI3K antibody binding site. Since the number of sites is limited, as more sites are occupied by PI3K from the sample, fewer sites are left to bind PI3K-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PI3K concentration in each sample is interpolated from this standard curve.

Intended Uses: This PI3K ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PI3K. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 118572681
• NCBI GeneID: 5295
• Molecular Weight: 42,838 Da
• NCBI Official Full Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• NCBI Official Symbol: PIK3R1
• NCBI Official Synonym Symbols: p85; AGM7; GRB1; IMD36; p85-ALPHA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
• UniProt Gene Name: PIK3R1
• UniProt Synonym Gene Names: GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
• UniProt Entry Name: P85A_HUMAN