Toxoplasma Gondii SAG1 protein Recombinant Protein | TOXGO SAG1 recombinant protein

Name: Toxoplasma Gondii SAG1 protein Recombinant Protein | TOXGO SAG1 recombinant protein
Brand: AAA Biotech
SKU: TOXOPLASMA-GONDII-SAG1-PROTEIN-RECOMBINANT-PROTEIN-TOXGO-SAG1-RECOMBINANT-PROTEIN
Availability: InStock

Toxoplasma Gondii SAG1 protein Recombinant

Applications

ELISA

Purity

GST affinity chromatography
Purity: >90%

Synonyms

Toxoplasma Gondii SAG1 protein; N/A; Toxoplasma Gondii SAG1 protein Recombinant; toxoplasma gondii; sag1; p30; TOXGO SAG1 recombinant protein

Ordering
Cat. Num. AAA76066

Host

E Coli

Purity/Purification

GST affinity chromatography
Purity: >90%

Form/Format

0.2um filtered solution: 50mM Tris-HCL pH 8.0, 60mM NaCl, 0.25% Sarkosyl, 10mM glutathione, 50% glycerol.

Concentration

4.8 mg/ml (varies by lot)

Sequence Length

336

Applicable Applications for TOXGO SAG1 recombinant protein

ELISA

Source

DNA sequence encoding immunodominant fragment 45-198AA of T.Gondii SAG1 protein was expressed in Escherichia Coli.

Preparation and Storage

This protein can be stored in working aliquots at 2 - 8°C for one month, or at -20°C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Related Product Information for TOXGO SAG1 recombinant protein

Recent studies indicate that SAG1 may play an important role in invasion of host cells. Unlike surface antigens of other protozoa, such as plasmodia and trypanosomes, SAG1 is a nonvariant antigen which is well conserved immunologically and at the sequence level. SAG1 elicits a robust immune response in infected animals, including humans, and immunization with purified SAG1 protects mice from a lethal parasite challenge. This immunity appears to be primarily mediated by CD8+ cells specific for SAG1

Product Categories/Family for TOXGO SAG1 recombinant protein

Viral Antigens

References

• evaluation of nested pcr targeting b1 gene with four npcrs (targeting the b1, sag2 and SAG1 gene) for detection of toxoplasma gondii genome in intra ocular fluids from toxoplasma retinochoroiditis patients in a tertiary eye hospital, chennai, india .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 857.
• reverse enzyme-linked immunosorbent assay using monoclonal antibodies against SAG1-related sequence, sag2a, and p97 antigens from toxoplasma gondii to detect specific immunoglobulin g (igg), igm, and iga antibodies in human sera .Clin. Vaccine Immunol., Aug 2008; 15: 1265 - 1271.
• azurin-like protein blocks invasion of toxoplasma gondii through potential interactions with parasite surface antigen SAG1 .Antimicrob. Agents Chemother., Feb 2008; 52: 402 - 408.
• the SAG1 toxoplasma gondii surface protein is not required for acute ocular toxoplasmosis in mice .Infect. Immun., Apr 2007; 75: 2079 - 2083.
• conformationally correct expression of membrane-anchored toxoplasma gondii SAG1 in the primitive protozoan giardia duodenalis .Infect. Immun., Feb 2002; 70: 1014 - 1016.

NCBI and Uniprot Product Information

NCBI GI #

74766948

UniProt Accession #

Q27298

Molecular Weight

Recombinant SAG1 contains an N terminal GST purification tag and migrates as an approximately 48 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE

NCBI Official Full Name

P30 (SAG1 protein)

UniProt Protein Name

P30

UniProt Gene Name

p30

UniProt Synonym Gene Names

SAG1

UniProt Entry Name

Q27298_TOXGO

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Product Notes
The TOXGO SAG1 p30 (Catalog #AAA76066) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Toxoplasma Gondii SAG1 protein can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the TOXGO SAG1 p30 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Toxoplasma Gondii SAG1 protein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.