Application Data
(Figure 5: ATAC-Seq Results are Consistent with Pre-indexed Assembled Tn5 Transposomes and Standard Tn5 Transposomes. 50,000 fresh K562 cells were assayed in ATAC-Seq with either Active Motif’s Pre-indexed Assembled Tn5 Transposomes (top four tracks) or standard, non-indexed Tn5 Assembled Transposomes (bottom track). ATAC-Seq results were consistent between both methods. Browser tracks are shown at Chr6:159,668,373-159,821,466.)
pA-Tn5 Transposase Recombinant Protein
pA-Tn5 Transposase
Synonyms
pA-Tn5 Transposase; N/A; pA-Tn5 Transposase recombinant protein
Host
E Coli
Additive
Glycerol
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(Figure 5: ATAC-Seq Results are Consistent with Pre-indexed Assembled Tn5 Transposomes and Standard Tn5 Transposomes. 50,000 fresh K562 cells were assayed in ATAC-Seq with either Active Motif’s Pre-indexed Assembled Tn5 Transposomes (top four tracks) or standard, non-indexed Tn5 Assembled Transposomes (bottom track). ATAC-Seq results were consistent between both methods. Browser tracks are shown at Chr6:159,668,373-159,821,466.)
Application Data
(Figure 5: ATAC-Seq Results are Consistent with Pre-indexed Assembled Tn5 Transposomes and Standard Tn5 Transposomes. 50,000 fresh K562 cells were assayed in ATAC-Seq with either Active Motif’s Pre-indexed Assembled Tn5 Transposomes (top four tracks) or standard, non-indexed Tn5 Assembled Transposomes (bottom track). ATAC-Seq results were consistent between both methods. Browser tracks are shown at Chr6:159,668,373-159,821,466.)
SDS-PAGE
(Figure 1: 12.5% SDS PAGE Gel of Recombinant Tn5 Transposase Protein (MW 53kDa).)
SDS-PAGE
(Figure 1: 12.5% SDS PAGE Gel of Recombinant Tn5 Transposase Protein (MW 53kDa).)
Related Product Information for pA-Tn5 Transposase recombinant protein
Recombinant pA-Tn5 Transposase protein is supplied at a concentration of 0.3 µg/µl in 50 mM HEPES-KOH pH 7.4, 100 mM NaCl, 50% glycerol, 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT.
Recombinant pA-Tn5 Transposase protein was expressed in E.coli as the full length protein (accession number AAC25438.1) with an N-terminal 3×DYKDDDDK and protein A tag. The molecular weight of the protein is 75 kDa.
Transposase for transposon Tn5 or Tnp , also known as Tn5, Mediates transposition of transposon Tn5 by a ‘cut and paste’ mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After nicking of the first DNA strand, excision of the transposon proceeds through a series of intermediates. The transposase then mediates the insertion of the transposon at a new site by strand transfer. The activity of the wild-type transposase is very low, and is further inhibited by dimerization with the transposase inhibitor (inh).
Recombinant pA-Tn5 Transposase protein was expressed in E.coli as the full length protein (accession number AAC25438.1) with an N-terminal 3×DYKDDDDK and protein A tag. The molecular weight of the protein is 75 kDa.
Transposase for transposon Tn5 or Tnp , also known as Tn5, Mediates transposition of transposon Tn5 by a ‘cut and paste’ mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After nicking of the first DNA strand, excision of the transposon proceeds through a series of intermediates. The transposase then mediates the insertion of the transposon at a new site by strand transfer. The activity of the wild-type transposase is very low, and is further inhibited by dimerization with the transposase inhibitor (inh).
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Product Notes
The pA-Tn5 Transposase (Catalog #AAA60348) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "pA-Tn5 Transposase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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