Pregnancy Associated Plasma Protein A (PAPPA) Recombinant Protein | PAPPA recombinant protein

Recombinant Pregnancy Associated Plasma Protein A (PAPPA)

Cat. Num. AAA134211

• Gene Names: Pappa; PAG1; PAPP-A; IGFBP-4ase; 8430414N03Rik
• Applications: Immunoprecipitation, ELISA, Western Blot, SDS-Page
• Purity: > 95%
• Synonyms: Pregnancy Associated Plasma Protein A (PAPPA); N/A; Recombinant Pregnancy Associated Plasma Protein A (PAPPA); PAPPA recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-FE QKTSIKDCGV YTPQGFLDQW ASNASVSHQD QQCPGWVVIG QPAASQVCRT KVIDLSEGIS QHAWYPCTIT YPYYHLPQTT FWLQTYFSQP MVAAAVIIHL VTDGTYYGDQ KQETISVQLL DTKDQSHDLG LHVLSCRNNP LIIPVVHDLS QPFYHSQAVH VSFSSPLVAI SGVALRSFDN FDPVTLSSCQ RGETYSPAEQ SCVHFACQAA DCPELAVGNA SLNCSSNHHY HGAQCTVSCQ TGYVLQIQRDDELIKSQVGP SITVTCTEGK WN
• Sequence Length: 1624

• Applicable Applications for PAPPA recombinant protein: IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Phe999~Asn1272 (Accession # Q8R4K8) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 5.8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-PAGE

Sequence

NCBI and Uniprot Product Information

• NCBI GI #: 59676577
• NCBI GeneID: 18491
• NCBI Accession #: NP_067337.1
• NCBI GenBank Nucleotide #: NM_021362.1
• UniProt Accession #: Q8R4K8; Q80VW3; Q80YY1; Q8K423; Q8R4K7; Q9ES06; Q9JK57
• Molecular Weight: 62.2kDa
• NCBI Official Full Name: pappalysin-1
• NCBI Official Synonym Full Names: pregnancy-associated plasma protein A
• NCBI Official Symbol: Pappa
• NCBI Official Synonym Symbols: PAG1; PAPP-A; IGFBP-4ase; 8430414N03Rik
• NCBI Protein Information: pappalysin-1; IGF-dependent IGFBP-4 protease; insulin-like growth factor-dependent IGF-binding protein 4 protease
• UniProt Protein Name: Pappalysin-1
• UniProt Gene Name: Pappa
• UniProt Synonym Gene Names: IGF-dependent IGFBP-4 protease; IGFBP-4ase; PAPP-A
• UniProt Entry Name: PAPP1_MOUSE