Application Data
PDE4D recombinant protein
PDE4D, Active
Gene Names
PDE4D; DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
Applications
PDE Assay
Purity
>85%
Synonyms
PDE4D; N/A; PDE4D, Active; PDE4D recombinant protein
Host
Sf9 insect cells using baculovirus
Purity/Purification
>85%
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCI, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1ug/ul (varies by lot)
Sequence Positions
225-end
Sequence Length
7783
Applicable Applications for PDE4D recombinant protein
PDE Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at –70°C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Ships on dry ice.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Ships on dry ice.
Application Data
Application Data
Application Data
(the specific activity of PDE4D was determined to be 1,250 nmol/min/mg as per activity assay protocol.)
Application Data
(the specific activity of PDE4D was determined to be 1,250 nmol/min/mg as per activity assay protocol.)
Related Product Information for PDE4D recombinant protein
Description: Recombinant human PDE4D (225-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE4D is a member of the phosphodiesterase (PDE) family that is cAMP-specific and has homology to the 'dunce' gene of Drosophila. PDE4D is expressed in both cytosolic and particulate fractions in the cell and Rolipram, a specific PDE4 inhibitor, inhibits and significantly lowers the IC50 for the cytosolic forms of the enzyme. TNFalpha upregulates the basal expression of PDE4D in HUVEC. PDE4D, which is not detected in untreated cells, accumulates beginning 4 hours after TNFalpha treatment and increased by 24 hours (1). Mice deficient in PDE4D exhibit delayed growth as well as reduced viability and female fertility (2).
Scientific Background: PDE4D is a member of the phosphodiesterase (PDE) family that is cAMP-specific and has homology to the 'dunce' gene of Drosophila. PDE4D is expressed in both cytosolic and particulate fractions in the cell and Rolipram, a specific PDE4 inhibitor, inhibits and significantly lowers the IC50 for the cytosolic forms of the enzyme. TNFalpha upregulates the basal expression of PDE4D in HUVEC. PDE4D, which is not detected in untreated cells, accumulates beginning 4 hours after TNFalpha treatment and increased by 24 hours (1). Mice deficient in PDE4D exhibit delayed growth as well as reduced viability and female fertility (2).
Product Categories/Family for PDE4D recombinant protein
References
1. Miro X, et al: Phosphodiesterases 4D and 7A splice variants in the response of HUVEC cells to TNF-alpha. Biochem. Biophys. Res. Commun. 274: 415-421, 2000. 2. Jin S-L C, et al: Impaired growth and fertility of cAMP-specific phosphodiesterase PDE4D-deficient mice. Proc. Nat. Acad. Sci. 96: 11998-12003, 1999.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~82 kDa
NCBI Official Full Name
Homo sapiens phosphodiesterase 4D (PDE4D), transcript variant 2, mRNA
NCBI Official Synonym Full Names
phosphodiesterase 4D
NCBI Official Symbol
PDE4D
NCBI Official Synonym Symbols
DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
NCBI Protein Information
cAMP-specific 3',5'-cyclic phosphodiesterase 4D
UniProt Protein Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4D
UniProt Gene Name
PDE4D
UniProt Synonym Gene Names
DPDE3
Similar Products
Product Notes
The PDE4D pde4d (Catalog #AAA73102) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 225-end. AAA Biotech's PDE4D can be used in a range of immunoassay formats including, but not limited to, PDE Assay. Researchers should empirically determine the suitability of the PDE4D pde4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE4D, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.