Application Data
PDE6A recombinant protein
PDE6A, Active
Gene Names
PDE6A; PDEA; RP43; CGPR-A
Applications
PDE Assay
Synonyms
PDE6A; N/A; PDE6A, Active; PDE6A recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
31-end
Sequence Length
5659
Applicable Applications for PDE6A recombinant protein
PDE Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for PDE6A recombinant protein
Description: Recombinant human PDE6A (31-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE6A encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A and is expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis (1). The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa (2).
Scientific Background: PDE6A encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A and is expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis (1). The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa (2).
Product Categories/Family for PDE6A recombinant protein
References
1. Pittler, S. J. et.al: Molecular characterization of human and bovine rod photoreceptor cGMP phosphodiesterase alpha- subunit and chromosomal localization of the human gene. Genomics 6: 272-283, 1990. 2. Danciger, M. et.al: Genetic mapping demonstrates that the alpha-subunit of retinal cGMP-phosphodiesterase is not the site of the rd mutation. Exp. Eye Res. 51: 185-189, 1990.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~120 kDa
NCBI Official Full Name
Homo sapiens phosphodiesterase 6A (PDE6A), mRNA
NCBI Official Synonym Full Names
phosphodiesterase 6A
NCBI Official Symbol
PDE6A
NCBI Official Synonym Symbols
PDEA; RP43; CGPR-A
NCBI Protein Information
rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
UniProt Protein Name
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
UniProt Gene Name
PDE6A
UniProt Synonym Gene Names
PDEA; GMP-PDE alpha
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Product Notes
The PDE6A pde6a (Catalog #AAA73105) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 31-end. AAA Biotech's PDE6A can be used in a range of immunoassay formats including, but not limited to, PDE Assay. Researchers should empirically determine the suitability of the PDE6A pde6a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE6A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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