Human Platelet Derived Growth Factor Subunit B ELISA Kit | PDGFB elisa kit

Human Platelet Derived Growth Factor Subunit B ELISA Kit

Cat. Num. AAA83575

• Gene Names: PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
• Reactivity: Human
• Synonyms: Platelet Derived Growth Factor Subunit B; N/A; Human Platelet Derived Growth Factor Subunit B ELISA Kit; PDGFB elisa kit

• Reactivity: Human
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL

• Intended Uses: This PDGFB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PDGFB. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PDGFB elisa kit

Principle of the Assay: PDGFB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDGFB antibody and an PDGFB-HRP conjugate. The assay sample and buffer are incubated together with PDGFB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDGFB concentration since PDGFB from samples and PDGFB-HRP conjugate compete for the anti-PDGFB antibody binding site. Since the number of sites is limited, as more sites are occupied by PDGFB from the sample, fewer sites are left to bind PDGFB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDGFB concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47678607
• NCBI GeneID: 5155
• UniProt Accession #: P01127; P78431; Q15354; Q6FHE7; Q9UF23; G3XAG8
• Molecular Weight: 25,502 Da
• NCBI Official Full Name: PDGFB
• NCBI Official Synonym Full Names: platelet-derived growth factor beta polypeptide
• NCBI Official Symbol: PDGFB
• NCBI Official Synonym Symbols: SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
• NCBI Protein Information: platelet-derived growth factor subunit B; becaplermin; PDGF, B chain; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor, beta polypeptide (oncogene SIS); platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
• UniProt Protein Name: Platelet-derived growth factor subunit B
• UniProt Gene Name: PDGFB
• UniProt Synonym Gene Names: PDGF2; SIS; PDGF subunit B
• UniProt Entry Name: PDGFB_HUMAN