PDGF Recombinant Protein | PDGFB recombinant protein
RECOMBINANT HUMAN PDGF BB HOMODIMER
Gene Names
PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
Applications
ELISA, Functional Assay, Western Blot
Purity
>98% by SDS PAGE and HPLC analysis
Synonyms
PDGF; N/A; RECOMBINANT HUMAN PDGF BB HOMODIMER; PDGFB recombinant protein
Host
E.coli
Purity/Purification
>98% by SDS PAGE and HPLC analysis
Form/Format
Purified recombinant protein - lyophilized
Concentration
Total protein concentration 0.1 mg/ml after reconstitution. (varies by lot)
Sequence Length
241
Applicable Applications for PDGFB recombinant protein
ELISA (EIA), Functional Assays (FN), Western Blot (WB)
Application Notes
ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;
Functional Assays: Minimum Dilution: 0.2ng/ml; Maximum Dilution: 10ng/ml;
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.Suggested working dilutions are given as a guide only.It is recommended that the user titrates the protein for use in their own systems using appropriate negative/positive controls.
Functional Assays: Minimum Dilution: 0.2ng/ml; Maximum Dilution: 10ng/ml;
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.Suggested working dilutions are given as a guide only.It is recommended that the user titrates the protein for use in their own systems using appropriate negative/positive controls.
Reconstitution
Reconstitute with 0.1ml distilled water.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Werecommends that the vial is gently mixed after reconstitution. After the initial reconstitution step, the product should be diluted to a working concentration using an aqueous buffer containg 0.1% BSA (or any albumin), or diluted in culture media containing serum, such as FCS.
Buffer Solution
30 mM Acetic Acid
Target Species
Human
Endotoxin Level
< 0.1 ng/ug
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Prior to reconstitution store at -20°C. Following reconstitution store at -20°C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.
Shelf Life: 3 months from date of reconstitution.
Related Product Information for PDGFB recombinant protein
MBS232238 is an E Coli-derived recombinant protein corresponding to mature full-length human Platelet-Derived Growth Factor BB homodimer (PDGF-BB), a potent mitogen for cells of mesenchymal origin. PDGF is composed of a dimer of two chains, known as the A chain and B chain. The dimer may present as either AA or BB homodimer, or as an AB heterodimer. MBS232238 has an ED50 of 1.0-3.0 ng/ml as determined by stimulation of the proliferation of Balb/c 3T3 cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Protein Molecular Weight: 24.3kD (218 amino acid sequence)
NCBI Official Full Name
platelet-derived growth factor subunit B isoform 1 preproprotein
NCBI Official Synonym Full Names
platelet-derived growth factor beta polypeptide
NCBI Official Symbol
PDGFB
NCBI Official Synonym Symbols
SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
NCBI Protein Information
platelet-derived growth factor subunit B; PDGF subunit B; PDGF, B chain; becaplermin; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor, beta polypeptide (oncogene SIS); proto-oncogene c-Sis
UniProt Protein Name
Platelet-derived growth factor subunit B
UniProt Gene Name
PDGFB
UniProt Synonym Gene Names
PDGF2; SIS; PDGF subunit B
UniProt Entry Name
PDGFB_HUMAN
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Product Notes
The PDGFB pdgfb (Catalog #AAA12302) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PDGF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Functional Assays (FN), Western Blot (WB). ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well; Functional Assays: Minimum Dilution: 0.2ng/ml; Maximum Dilution: 10ng/ml; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.Suggested working dilutions are given as a guide only.It is recommended that the user titrates the protein for use in their own systems using appropriate negative/positive controls. Researchers should empirically determine the suitability of the PDGFB pdgfb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDGF, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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