Monkey Pigment Epithelium Derived Factor ELISA Kit | PEDF elisa kit

Monkey Pigment Epithelium Derived Factor ELISA Kit

Cat. Num. AAA89612

• Gene Names: SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35
• Reactivity: Monkey
• Synonyms: Pigment Epithelium Derived Factor; N/A; Monkey Pigment Epithelium Derived Factor ELISA Kit; PEDF elisa kit

• Reactivity: Monkey
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL

• Intended Uses: This PEDF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey PEDF. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PEDF elisa kit

Principle of the assay: PEDF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PEDF. Standards or samples are then added to the microtiter plate wells and PEDF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PEDF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PEDF are added to each well to “sandwich” the PEDF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PEDF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PEDF concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PEDF elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 39725934
• NCBI GeneID: 5176
• NCBI Accession #: NP_002606.3
• NCBI GenBank Nucleotide #: NM_002615.5
• Molecular Weight: 46,312 Da
• NCBI Official Full Name: pigment epithelium-derived factor
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• NCBI Official Symbol: SERPINF1
• NCBI Official Synonym Symbols: OI6; OI12; PEDF; EPC-1; PIG35
• NCBI Protein Information: pigment epithelium-derived factor; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• UniProt Protein Name: Pigment epithelium-derived factor
• UniProt Gene Name: SERPINF1
• UniProt Synonym Gene Names: PEDF; PIG35; PEDF
• UniProt Entry Name: PEDF_HUMAN