Sulf2 peptide
Sulf2
Gene Names
SULF2; HSULF-2
Synonyms
Sulf2; N/A; Extracellular Sulfatase-2; HSulf-2; Sulf2 peptide
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Sequence Length
870
Comment
Blocking peptide for use with Sulf-2 antibodies
Instruction
Incubate antibody neat with at least a 50 fold stoichiometric excess of blocking peptide at 37 deg C for 20 minutes (molecular weights of peptide and antibody are ~2.5 kDa and ~160 kDa, respectively). Antibody can then be diluted to a concentration suitable for Western blot. Example: 10 ?l or 10 ?g of Exalpha's rabbit anti-Sulf-2 is added to 10 ?g of blocking peptide for a total volume of 20 ?l. The mixture is allowed to incubate for 20 minutes at 37 deg C prior to dilution in suitable buffer (for Western blot, etc.).
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Products are stable for one year from purchase when stored properly.
Products are stable for one year from purchase when stored properly.
Related Product Information for Sulf2 peptide
Sulf-2 exhibits arylsulfatase activity and highly specific endoglucosamine -6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.
Heparan sulfate proteoglycans act as co-receptors for numerous heparinbinding growth factors and cytokines and are key regulators of cell signaling. Growth factor binding to heparan sulfate results in mitogenic activity only when specific structural features are present within the heparan sulfate chains. These features include sulfation at specific positions within a disaccharide (N, 2-O, 3-O, 6-O) by the enzymes that regulate heparan sulfate synthesis inside the Golgi. Following synthesis and expression, heparan sulfate can be structurally and functionally modified within the extracellular compartment. Enzymes known to have these effects are heparanase, which cleaves heparan sulfate chains into smaller biologically active fragments, and the heparan sulfate 6-Oendosulfatases (Sulfs). The Sulfs represent a new and novel family of enzymes that are secreted via the Golgi and subsequently localized to the cell surface or may be released into the extracellular matrix. Sulf-1 enzyme is required for Wnt-mediated signaling in developing quail muscle. Sulf1 was shown (in quial) to restore bone morphogenetic protein signaling in cells by releasing the functional inhibitor, Noggin, from cell surfaces. Quail Sulf1 can also inhibit growth factor signaling, the removal of the 6-O-sulfation is required for the formation of the FGF HS FR1c ternary complex blocking FGF2 signaling.
Analysis of human tumor tissue and tumor cell lines suggests that HSulf-1 is misregulated in cancers. HSulf-1 is found in a variety of normal tissues but is down-regulated in tumor cell lines originating from ovarian, breast, pancreatic, renal, and hepatocellular carcinoma.
Heparan sulfate proteoglycans act as co-receptors for numerous heparinbinding growth factors and cytokines and are key regulators of cell signaling. Growth factor binding to heparan sulfate results in mitogenic activity only when specific structural features are present within the heparan sulfate chains. These features include sulfation at specific positions within a disaccharide (N, 2-O, 3-O, 6-O) by the enzymes that regulate heparan sulfate synthesis inside the Golgi. Following synthesis and expression, heparan sulfate can be structurally and functionally modified within the extracellular compartment. Enzymes known to have these effects are heparanase, which cleaves heparan sulfate chains into smaller biologically active fragments, and the heparan sulfate 6-Oendosulfatases (Sulfs). The Sulfs represent a new and novel family of enzymes that are secreted via the Golgi and subsequently localized to the cell surface or may be released into the extracellular matrix. Sulf-1 enzyme is required for Wnt-mediated signaling in developing quail muscle. Sulf1 was shown (in quial) to restore bone morphogenetic protein signaling in cells by releasing the functional inhibitor, Noggin, from cell surfaces. Quail Sulf1 can also inhibit growth factor signaling, the removal of the 6-O-sulfation is required for the formation of the FGF HS FR1c ternary complex blocking FGF2 signaling.
Analysis of human tumor tissue and tumor cell lines suggests that HSulf-1 is misregulated in cancers. HSulf-1 is found in a variety of normal tissues but is down-regulated in tumor cell lines originating from ovarian, breast, pancreatic, renal, and hepatocellular carcinoma.
Product Categories/Family for Sulf2 peptide
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
100455 Da
NCBI Official Full Name
extracellular sulfatase Sulf-2 isoform a
NCBI Official Synonym Full Names
sulfatase 2
NCBI Official Symbol
SULF2
NCBI Official Synonym Symbols
HSULF-2
NCBI Protein Information
extracellular sulfatase Sulf-2
UniProt Protein Name
Extracellular sulfatase Sulf-2
UniProt Gene Name
SULF2
UniProt Synonym Gene Names
KIAA1247; hSulf-2
UniProt Entry Name
SULF2_HUMAN
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Product Notes
The Sulf2 sulf2 (Catalog #AAA60513) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Sulf2, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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