Rat Pepsinogen A ELISA Kit | PGA elisa kit
Rat Pepsinogen A ELISA Kit
Reactivity
Rat
Synonyms
Pepsinogen A; N/A; Rat Pepsinogen A ELISA Kit; PGA elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/ml
Intended Uses
This PGA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PGA. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for PGA elisa kit
Principle of the assay: PGA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGA antibody and an PGA-HRP conjugate. The assay sample and buffer are incubated together with PGA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGA concentration since PGA from samples and PGA-HRP conjugate compete for the anti-PGA antibody binding site. Since the number of sites is limited, as more sites are occupied by PGA from the sample, fewer sites are left to bind PGA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41,993 Da
NCBI Official Full Name
pepsinogen A
NCBI Official Synonym Full Names
pepsinogen 5, group I (pepsinogen A)
NCBI Official Symbol
PGA5
NCBI Protein Information
pepsin A-5; pepsinogen-5; pepsinogen A5
UniProt Protein Name
Pepsin A-5
UniProt Gene Name
PGA5
UniProt Entry Name
PEPA5_HUMAN
