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Standard Curve (Sample)

Human Phosphoglycerate Mutase 2 ELISA Kit | PGAM2 elisa kit

Human Phosphoglycerate Mutase 2 ELISA Kit

Gene Names
PGAM2; GSD10; PGAMM; PGAM-M
Reactivity
Human
Synonyms
Phosphoglycerate Mutase 2; Human Phosphoglycerate Mutase 2 ELISA Kit; PGAM2 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PGM2. No significant cross-reactivity or interference between PGM2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PGM2 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive or Sandwich
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Standard Curve (Sample)
Related Product Information for PGAM2 elisa kit
Principle of the Assay: PGM2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PGM2. Standards or samples are then added to the microtiter plate wells and PGM2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PGM2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PGM2 are added to each well to "sandwich" the PGM2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PGM2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGM2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This PGM2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PGM2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,766 Da
NCBI Official Full Name
phosphoglycerate mutase 2
NCBI Official Synonym Full Names
phosphoglycerate mutase 2 (muscle)
NCBI Official Symbol
PGAM2
NCBI Official Synonym Symbols
GSD10; PGAMM; PGAM-M
NCBI Protein Information
phosphoglycerate mutase 2; BPG-dependent PGAM 2; phosphoglycerate mutase isozyme M; muscle-specific phosphoglycerate mutase
UniProt Protein Name
Phosphoglycerate mutase 2
UniProt Gene Name
PGAM2
UniProt Synonym Gene Names
PGAMM; PGAM-M
UniProt Entry Name
PGAM2_HUMAN

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Product Notes

The Human PGAM2 pgam2 (Catalog #AAA16587) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA16587 ELISA Kit recognizes Human PGAM2. It is sometimes possible for the material contained within the vial of "Phosphoglycerate Mutase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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